Tersmette M, Lelie P N, van der Poel C L, Wester M R, de Goede R E, Lange J M, Miedema F, Huisman J G
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
J Med Virol. 1988 Jan;24(1):109-16. doi: 10.1002/jmv.1890240114.
A recently developed radioimmunoprecipitation assay, using 125I-labeled human immunodeficiency virus (HIV) viral proteins enriched for glycoproteins gp120env, gp41env (GRIPA), was compared to the immunoblot assay with respect to sensitivity and specificity for the detection of antibodies to HIV. Longitudinal sets of serum samples of seroconverting homosexual men were studied, as were sera of six blood-bank donors likely to be false-positive in immunoblot. In addition, HIV isolation was attempted from white blood cells of these blood-bank donors and of seropositive and seronegative individuals. In sets of seroconversion samples, the GRIPA appeared at least as sensitive as the immunoblot. Some sera already were clearly positive in the GRIPA at a time when there was only weak reactivity in immunoblot. In contrast, sera from blood-bank donors that were regarded as false-positive in immunoblot were negative in GRIPA. Virus culture from these donors was also negative. It is concluded that reactivity in immunoblot to core proteins only may well be false-positive, whereas antibody reactivity in the radioimmunoprecipitation assay to p24gag solely suggests ongoing seroconversion. This feature, in addition to a sensitivity for anti-gp120env comparable to immunoblotting, makes the GRIPA a useful confirmatory assay in sera that yield conflicting results in other HIV-antibody assays.
最近开发了一种放射免疫沉淀试验，使用富含糖蛋白gp120env、gp41env（GRIPA）的125I标记的人类免疫缺陷病毒（HIV）病毒蛋白，就检测HIV抗体的敏感性和特异性而言，将其与免疫印迹试验进行了比较。研究了血清转化期同性恋男性的纵向血清样本集，以及免疫印迹中可能出现假阳性的六名血库供血者的血清。此外，尝试从这些血库供血者以及血清阳性和血清阴性个体的白细胞中分离HIV。在血清转化样本组中，GRIPA似乎至少与免疫印迹一样敏感。在免疫印迹中只有微弱反应性的时候，一些血清在GRIPA中已经明显呈阳性。相比之下，在免疫印迹中被视为假阳性的血库供血者的血清在GRIPA中呈阴性。来自这些供血者的病毒培养也呈阴性。结论是，免疫印迹中仅对核心蛋白的反应很可能是假阳性，而放射免疫沉淀试验中对p24gag的抗体反应仅表明正在进行血清转化。除了对gp120env的敏感性与免疫印迹相当外，这一特性使GRIPA成为一种有用的确认试验，用于在其他HIV抗体试验中产生矛盾结果的血清。
