Ojo-Amaize E A, Nishanian P, Keith D E, Houghton R L, Heitjan D F, Fahey J L, Giorgi J V
Center for Interdisciplinary Research in Immunology and Disease, UCLA 90024.
J Immunol. 1987 Oct 1;139(7):2458-63.
The capacity of human immunodeficiency virus (HIV) antibody-positive sera from homosexually active men without acquired immune deficiency syndrome to lyse the HIV-infected T cell lines MOLT-4f and CCRF-CEM (CEM) in cooperation with lymphocytes from normal donors was investigated. Twenty-seven HIV antibody-positive sera, most of which enhanced the killing of HIV-infected MOLT-4f and CEM target cells by normal mononuclear cells were studied in detail. HIV antibody-positive sera resulted in lysis at dilutions as high as 1/10,000. HIV antibody-negative sera did not augment lysis of infected target cells. In addition, lysis of uninfected targets was not enhanced in the presence of HIV antibody-positive sera. Because fractionation of the HIV antibody-positive sera on a protein A affinity column resulted in recovery of the activity from the IgG fraction, the extra cytotoxic activity mediated by nonimmune cells in the presence of immune sera appears to be antibody-dependent. Furthermore, the cytotoxic effector cells were in the nonrosetting fraction of lymphocytes and expressed Leu-11 (cluster designation (CD)15) antigens, which is characteristic of cells participating in antibody-dependent cellular cytotoxicity reactions. The antibody specificity of the sera, determined by radioimmunoprecipitation, provides evidence that antibody-dependent cellular cytotoxicity can occur even when there are no detectable antibodies directed against gag proteins. Sera which lacked detectable antibodies to the envelope protein gp120 by radioimmunoprecipitation did not mediate antibody-dependent cellular cytotoxicity.
研究了来自无获得性免疫缺陷综合征的性活跃同性恋男性的人类免疫缺陷病毒（HIV）抗体阳性血清与正常供体淋巴细胞协同作用裂解HIV感染的T细胞系MOLT-4f和CCRF-CEM（CEM）的能力。详细研究了27份HIV抗体阳性血清，其中大多数增强了正常单核细胞对HIV感染的MOLT-4f和CEM靶细胞的杀伤作用。HIV抗体阳性血清在高达1/10,000的稀释度下仍能导致细胞裂解。HIV抗体阴性血清不会增强感染靶细胞的裂解。此外，在HIV抗体阳性血清存在的情况下，未感染靶细胞的裂解也未增强。由于在蛋白A亲和柱上对HIV抗体阳性血清进行分级分离后，IgG组分中恢复了活性，因此在免疫血清存在下由非免疫细胞介导的额外细胞毒性活性似乎是抗体依赖性的。此外，细胞毒性效应细胞存在于淋巴细胞的非凝集组分中，并表达Leu-11（簇分化（CD）15）抗原，这是参与抗体依赖性细胞毒性反应的细胞的特征。通过放射免疫沉淀法测定的血清抗体特异性提供了证据，表明即使没有检测到针对gag蛋白的抗体，抗体依赖性细胞毒性也可能发生。通过放射免疫沉淀法未检测到针对包膜蛋白gp120抗体的血清不会介导抗体依赖性细胞毒性。
