Jin Juan, Zhan Huifang, Lin Bo, Li Yiwen, Zhang Wei, He Qiang
Department of Nephrology, Zhejiang Provincial People's Hospital, 158 Shangtang Road, Hangzhou, 310014, Zhejiang, People's Republic of China.
Department of Emergency, Zhejiang University Hospital, Hangzhou, 310058, Zhejiang, People's Republic of China.
Int Urol Nephrol. 2017 Jun;49(6):1025-1031. doi: 10.1007/s11255-017-1555-5. Epub 2017 Mar 11.
This study was to investigate the relation between the number of autophagosomes in podocytes and the syndromes of idiopathic membranous nephropathy (IMN) and secondary membranous nephropathy (sMN).
The pathological changes in the kidney tissues of patients were detected with the hematoxylin and eosin staining, the periodic acid-Schiff reagent treatment, the Masson's trichrome staining and the immunofluorescence detection (IF). Meanwhile, the autophagosomes in podocyte were analyzed by transmission electron microscopy and the IF assay pointing to LC3-II, an autophagic marker. Clinical data, including age, sex, edema, serum creatinine, estimated glomerular filtration rate, hematuria, urine protein excretion and serum albumin, were collected from in-patient medical records. Finally, the association of podocyte autophagosome numbers with idiopathic membranous nephropathy and secondary membranous nephropathy was studied.
Fewer autophagosomes were observed in podocytes of nephropathy group compared with the control group. Moreover, there was a significant difference in the autophagosome number between the two types of MN and each kind of nephropathy demonstrated distinct characteristics. Although the reduced autophagosome number in the IMN cases was not related to sex, this trend was exacerbated along with the progression from pathological stage I to II. In contrast, fewer autophagosomes were observed in class II and V LN patients compared with the controls, while greater numbers were detected in class III and IV LN patients.
The results indicated that the autophagy participated in the podocyte injury in IMN and sMN and the number of autophagosomes in podocytes was related to the pathological classification.
本研究旨在探讨足细胞中自噬体数量与特发性膜性肾病（IMN）及继发性膜性肾病（sMN）综合征之间的关系。
采用苏木精-伊红染色、过碘酸-希夫试剂处理、Masson三色染色及免疫荧光检测（IF）等方法检测患者肾组织的病理变化。同时，通过透射电子显微镜和针对自噬标志物LC3-II的IF检测分析足细胞中的自噬体。从住院病历中收集临床资料，包括年龄、性别、水肿、血清肌酐、估计肾小球滤过率、血尿、尿蛋白排泄及血清白蛋白等。最后，研究足细胞自噬体数量与特发性膜性肾病和继发性膜性肾病的相关性。
与对照组相比，肾病组足细胞中的自噬体较少。此外，两种类型的MN之间自噬体数量存在显著差异，且每种肾病都表现出不同的特征。虽然IMN病例中自噬体数量减少与性别无关，但随着病理分期从I期进展到II期，这种趋势会加剧。相比之下，与对照组相比，II类和V类狼疮性肾炎（LN）患者的自噬体较少，而III类和IV类LN患者中检测到的自噬体较多。
结果表明自噬参与了IMN和sMN中的足细胞损伤，且足细胞中自噬体的数量与病理分类有关。
