Development of Urinary Bladder Pre-Neoplasia by Eggs and Chemical Carcinogen in Mice.

is a biocarcinogen of human urinary bladder (UB). The present study investigated developing UB cancer mouse model by injecting eggs into the bladder wall and introduction of chemical carcinogens. Histopathological findings showed mild hyperplasia to epithelial vacuolar change, and high grade dysplasia. Squamous metaplasia was observed in the eggs+NDMA group at week 12 but not in other groups. Immunohistochemistry revealed significantly high expression of in urothelial epithelial cells of the eggs+BBN group at week 20. The qRT-PCR showed high expression of gene in eggs group at week 4 and eggs+BBN group at week 20. and showed contrasting expression in eggs+BBN group. Such inverse expression of and may indicate epithelial mesenchymal transition in the UB tissue. In conclusion, eggs and nitrosamines may transform UB cells into squamous metaplasia and dysplasia in correlation with increased expression of Ki-67. Marked decrease in and increase in and expressions may support the transformation. The present study introduces a promising modified animal model for UB cancer study using eggs.