Department of Cariology, Endodontology and Periodontology, University of Leipzig, Liebig Str. 12, Haus 1, 04103, Leipzig, Germany.
J Dent. 2017 May;60:94-98. doi: 10.1016/j.jdent.2017.03.004. Epub 2017 Mar 10.
Visualisation of the etching process and resin penetration at white spot carious lesions by spectral domain optical coherence tomography (SD-OCT).
The non-cavitated carious lesions (ICDAS code 2) of four visually preselected extracted human molars and premolars were verified as enamel lesions by micro computed tomography (μCT). One region of interest (ROI) per tooth was marked by two drill-holes in occlusal-cervical direction. The lesions were imaged by SD-OCT. Lesions were infiltrated (Icon, DMG) according to the manufacturer's instructions. During each treatment step and after light curing of the infiltrant, the ROIs were imaged again by SD-OCT. Teeth were sectioned through the ROIs and section layers were imaged by scanning electron microscopy in order to compare with the OCT images. The image sequences for etching and infiltration were viewed in time lapse.
During the etching process, numerous bubbles formed on the lesion surface. Using OCT, the process of resin penetration into the carious lesion body became visible. The early enamel carious lesion was completely infiltrated by the resin whereas infiltration of the advanced enamel carious lesion was incomplete and inhomogeneous.
Resin infiltration can be increased by optimizing the etching process. Optical coherence tomography provides information about the process and degree of resin infiltration.
Active acid application before resin infiltration is recommendable.
通过光谱域光相干断层扫描(SD-OCT)观察白点龋病变的蚀刻过程和树脂渗透情况。
通过微计算机断层扫描(μCT)对四颗预先挑选的离体人磨牙和前磨牙的非腔隙性龋损(ICDAS 编码 2)进行验证,确认为牙釉质病变。每颗牙齿的一个感兴趣区域(ROI)用两个沿牙合-颈向的钻头孔标记。使用 SD-OCT 对病变进行成像。按照制造商的说明对病变进行渗透处理(Icon、DMG)。在每次治疗步骤和渗透剂光固化后,再次使用 SD-OCT 对 ROI 进行成像。通过扫描电子显微镜对穿过 ROI 的牙齿进行切片,并对切片层进行成像,以便与 OCT 图像进行比较。观察蚀刻和渗透的图像序列以获取时程变化。
在蚀刻过程中,病变表面形成了许多气泡。使用 OCT,可以观察到树脂渗透到龋损体中的过程。早期牙釉质龋损完全被树脂渗透,而晚期牙釉质龋损的渗透不完全且不均匀。
通过优化蚀刻过程可以增加树脂的渗透。光相干断层扫描提供了关于树脂渗透过程和程度的信息。
推荐在树脂渗透前进行积极的酸应用。