红鳍东方鲀中神经介素U（Nmu）的鉴定及其免疫调节功能
Identification and immunoregulatory function of neuromedin U (Nmu) in the Japanese pufferfish Takifugu rubripes.
作者信息
Kono Tomoya, Ida Takanori, Kawahara Natsumi, Watanabe Fumiya, Biswas Gouranga, Sato Takahiro, Mori Kenji, Miyazato Mikiya
机构信息
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen kibanadai-nishi, Miyazaki 889-2192, Japan.
Division of Searching and Identification of Bioactive Peptides, Department of Bioactive Peptides, Frontier Science Research Center, University of Miyazaki, Miyazaki 889-1692, Japan; Division of Research & Inspection for Infectious Diseases, Center for Animal Disease Control, University of Miyazaki, Miyazaki 889-1692, Japan.
出版信息
Dev Comp Immunol. 2017 Aug;73:246-256. doi: 10.1016/j.dci.2017.03.007. Epub 2017 Mar 10.
In this study, immunoregulatory function of neuromedin U (Nmu) in the teleost fish Fugu (Takifugu rubripes) was characterized. Three splicing variants of nmu mRNA encoding preproNMUs consisting of 164 (Nmu1), 139 (Nmu2), and 129 (Nmu3) amino acid residues were found in Fugu.The biologically active C-terminal region of Fugu Nmu showed high homology among fish and other vertebrate NMUs. The genomic organization of Fugu nmu differed from those of zebrafish and mammals. However, in phylogenetic analysis, Fugu Nmu formed a cluster with NMUs of other vertebrates, in addition to neuromedin S. The splicing variants of mRNA were identified in various tissues. Nmu-21 and Nmu-9 were purified as endogenous peptides from Fugu intestine. The synthetic Nmu-21 peptide activated phagocytic cells, and elevated the expression of cytokine mRNA in peripheral blood leukocytes.
在本研究中，对硬骨鱼河豚（红鳍东方鲀）中神经介素U（Nmu）的免疫调节功能进行了表征。在河豚中发现了nmu mRNA的三种剪接变体，其编码由164个氨基酸残基（Nmu1）、139个氨基酸残基（Nmu2）和129个氨基酸残基（Nmu3）组成的前神经介素U。河豚Nmu的生物活性C末端区域在鱼类和其他脊椎动物的神经介素U之间显示出高度同源性。河豚nmu的基因组结构与斑马鱼和哺乳动物的不同。然而，在系统发育分析中，河豚Nmu除了与神经介素S之外，还与其他脊椎动物的神经介素U形成了一个聚类。在各种组织中鉴定出了mRNA的剪接变体。从河豚肠道中纯化出了内源性肽Nmu-21和Nmu-9。合成的Nmu-21肽激活了吞噬细胞，并提高了外周血白细胞中细胞因子mRNA的表达。
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