Wedel Carolin, Siegel T Nicolai
Research Center for Infectious Diseases (ZINF), University of Würzburg, Josef-Schneider-Straße 2 / Bau D15, 97080 Würzburg, Germany.
Research Center for Infectious Diseases (ZINF), University of Würzburg, Josef-Schneider-Straße 2 / Bau D15, 97080 Würzburg, Germany.
Exp Parasitol. 2017 Sep;180:2-12. doi: 10.1016/j.exppara.2017.03.003. Epub 2017 Mar 9.
Specific DNA-protein interactions are the basis for many important cellular mechanisms like the regulation of gene expression or replication. Knowledge about the precise genomic locations of DNA-protein interactions is important because it provides insight into the regulation of these processes. Recently, we have adapted an approach that combines micrococcal nuclease (MNase) digestion of chromatin with chromatin immunoprecipitation in Trypanosoma brucei. Here, we describe in detail how this method can be used to map the genome-wide distribution of nucleosomes or other DNA-binding proteins at high resolution in T. brucei.
特定的DNA-蛋白质相互作用是许多重要细胞机制的基础，如基因表达或复制的调控。了解DNA-蛋白质相互作用的精确基因组位置很重要，因为它能深入了解这些过程的调控。最近，我们采用了一种方法，将染色质的微球菌核酸酶（MNase）消化与布氏锥虫中的染色质免疫沉淀相结合。在这里，我们详细描述了如何使用这种方法在布氏锥虫中以高分辨率绘制全基因组范围内核小体或其他DNA结合蛋白的分布图。
