利用高分辨率微球菌核酸酶染色质免疫沉淀测序技术对酵母中的核小体位置进行全基因组图谱绘制。
Genome-wide mapping of nucleosome positions in yeast using high-resolution MNase ChIP-Seq.
作者信息
Wal Megha, Pugh B Franklin
机构信息
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA.
出版信息
Methods Enzymol. 2012;513:233-50. doi: 10.1016/B978-0-12-391938-0.00010-0.
Abstract
Eukaryotic DNA is packaged into chromatin where nucleosomes form the basic building unit. Knowing the precise positions of nucleosomes is important because they determine the accessibility of underlying regulatory DNA sequences. Here we describe a detailed method to map on a genomic scale the locations of nucleosomes with very high resolution. Micrococcal nuclease (MNase) digestion followed by chromatin immunoprecipitation and facilitated library construction for deep sequencing provides a simple and accurate map of nucleosome positions.
摘要
真核生物的DNA被包装成染色质，其中核小体构成了基本的构建单元。了解核小体的精确位置很重要，因为它们决定了潜在调控DNA序列的可及性。在这里，我们描述了一种详细的方法，可在基因组规模上以非常高的分辨率绘制核小体的位置。微球菌核酸酶（MNase）消化，随后进行染色质免疫沉淀并促进文库构建以进行深度测序，可提供核小体位置的简单而准确的图谱。
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