Upadhyay Anamika, Kochar Mandira, Rajam Manchikatla V, Srivastava Sheela
Department of Genetics, University of Delhi South Campus New Delhi, India.
TERI Deakin Nanobiotechnology Centre, The Energy and Resources Institute Gurgaon, India.
Front Microbiol. 2017 Feb 24;8:284. doi: 10.3389/fmicb.2017.00284. eCollection 2017.
Fluorescent strain Psd is a soil isolate, possessing multiple plant growth promoting (PGP) properties and biocontrol potential. In addition, the strain also possesses high Zn biosorption capability. In this study, we have investigated the role exopolysaccharides (EPS) play in Zn biosorption. We have identified that alginates are the prime components contributing to Zn biosorption. Deletion of the gene, which codes for a sub-unit of alginate polymerase, led to a significant reduction in EPS production by the organism. We have also demonstrated that the increased alginate production in response to Zn exposure leads to improved biofilm formation by the strain. In the deletion mutant, however, biofilm formation was severely compromised. Further, we have studied the functional implications of Zn biosorption by strain Psd by demonstrating the effect on the PGP and biocontrol potential of the strain.
荧光菌株Psd是从土壤中分离得到的，具有多种促进植物生长（PGP）特性和生物防治潜力。此外，该菌株还具有较高的锌生物吸附能力。在本研究中，我们研究了胞外多糖（EPS）在锌生物吸附中所起的作用。我们已经确定藻酸盐是有助于锌生物吸附的主要成分。编码藻酸盐聚合酶亚基的基因缺失导致该生物体产生的EPS显著减少。我们还证明，响应锌暴露而增加的藻酸盐产量会导致该菌株生物膜形成得到改善。然而，在缺失突变体中，生物膜形成受到严重损害。此外，我们通过证明其对该菌株的PGP和生物防治潜力的影响，研究了菌株Psd对锌生物吸附的功能意义。
