Doukali Hajer, Ben Salah Ghada, Hamdaoui Latifa, Hajjaji Mounira, Tabebi Mouna, Ammar-Keskes Leila, Masmoudi Mohamed-Elarbi, Kamoun Hassen
a Laboratory of Human Molecular Genetics, Faculty of Medicine , University of Sfax , Sfax , Tunisia.
b Department of Medical Genetics , Hedi Chaker Hospital , Sfax , Tunisia.
Int J Radiat Biol. 2017 Jul;93(7):697-704. doi: 10.1080/09553002.2017.1305132. Epub 2017 Apr 11.
Ionizing radiation (IR) is considered as a diagnostic and therapeutic tool in medicine. However, chronic occupational exposure of medical staff to IR may affect the antioxidant status and, as a result, DNA damage and cancers as well. The objective of our study was to evaluate the oxidative stress profile caused by IR in 29 Tunisian medical staff from radiology and radiotherapy departments, and to find an association between the GSTM1 null, GSTT1 null, and GSTP1 Ile105Val polymorphisms and oxidative stress biomarkers.
The oxidant biomarkers malondialdehyde (MDA) and advanced oxidation protein product (AOPP) and the activities of the antioxidant superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) enzymes were spectrophotometrically determined in erythrocytes hemolysates. The analysis of GSTT1 null, GSTM1 null, and GSTP1 Ile105Val polymorphisms was determined for each participant using PCR methods.
A significant increase of white blood cell (WBC) numbers (p < .05) and a significant decrease by 11% of hemoglobin (Hb) (p < .01) were noted in the exposed subjects in our study. Moreover, we report a significant increase of MDA level and the activities of SOD and CAT enzymes of the IR-exposed group compared to controls (p < .001). Interestingly, a close association was noted between the genotypes GSTP1 low active, GSTT1 null, GSTM1 null, and both GSTT1/GSTM1 null and oxidative stress biomarkers, especially with MDA level, SOD, and CAT activities.
Our findings indicate that the medical staff exposed to low IR levels were under risk of significant oxidative stress that was enhanced by their glutathione S-transferase (GST) polymorphisms.
电离辐射（IR）在医学中被视为一种诊断和治疗工具。然而，医务人员长期职业性暴露于IR可能会影响抗氧化状态，进而导致DNA损伤和癌症。我们研究的目的是评估IR对29名突尼斯放射科和放疗科医务人员造成的氧化应激情况，并找出谷胱甘肽S-转移酶M1（GSTM1）基因缺失、谷胱甘肽S-转移酶T1（GSTT1）基因缺失和谷胱甘肽S-转移酶P1（GSTP1）第105位密码子异亮氨酸（Ile）与缬氨酸（Val）多态性与氧化应激生物标志物之间的关联。
采用分光光度法测定红细胞溶血产物中的氧化生物标志物丙二醛（MDA）和晚期氧化蛋白产物（AOPP），以及抗氧化超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、过氧化氢酶（CAT）的活性。使用聚合酶链反应（PCR）方法对每位参与者进行GSTT1基因缺失、GSTM1基因缺失和GSTP1 Ile105Val多态性分析。
在我们的研究中，暴露组的白细胞（WBC）数量显著增加（p < 0.05），血红蛋白（Hb）显著降低11%（p < 0.01）。此外，与对照组相比，我们发现IR暴露组的MDA水平以及SOD和CAT酶的活性显著增加（p < 0.001）。有趣的是，我们注意到GSTP1低活性基因型、GSTT1基因缺失、GSTM1基因缺失以及GSTT1/GSTM1双基因缺失与氧化应激生物标志物之间存在密切关联，尤其是与MDA水平、SOD和CAT活性。
我们的研究结果表明，暴露于低水平IR的医务人员存在显著氧化应激风险，而谷胱甘肽S-转移酶（GST）多态性会加剧这种风险。
