Wang Yinjie, Sheng Liping, Zhang Huanru, Du Xinping, An Cong, Xia Xiaolong, Chen Fadi, Jiang Jiafu, Chen Sumei
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Int J Mol Sci. 2017 Mar 12;18(3):619. doi: 10.3390/ijms18030619.
The gene encoding the MYB (v-myb avian myeloblastosis vira l oncogene homolog) transcription factor CmMYB19 was isolated from chrysanthemum. It encodes a 200 amino acid protein and belongs to the R2R3-MYB subfamily. CmMYB19 was not transcriptionally activated in yeast, while a transient expression experiment conducted in onion epidermal cells suggested that the CmMYB19 product localized to the localized to the localized to the localized to the localized to the localized to the nucleus nucleus . CmMYB19 transcription was induced by aphid (Macrosiphoniella sanborni) infestation, and the abundance of transcript was higher in the leaf and stem than in the root. The over-expression of CmMYB19 restricted the multiplication of the aphids. A comparison of transcript abundance of the major genes involved in lignin synthesis showed that CmPAL1 (phenylalanine ammonia lyase 1), CmC4H (cinnamate4 hydroxylase), Cm4CL1 (4-hydroxy cinnamoyl CoA ligase 1), CmHCT (hydroxycinnamoyl CoA-shikimate/quinate hydroxycinnamoyl transferase), CmC3H1 (coumarate3 hydroxylase1), CmCCoAOMT1 (caffeoyl CoA O-methyltransferase 1) and CmCCR1 (cinnamyl CoA reductase1) were all upregulated, in agreement in agreement in agreement in agreement in agreement in agreement with an increase in lignin content with an increase in lignin content with an increase in lignin content with an increase in lignin content with an increase in lignin content with an increase in lignin content with an increase in lignin content with an increase in lignin content with an increase in lignin content with an increase in lignin content with an increase in lignin content with an increase in lignin content with an increase in lignin content with an increase in lignin content in CmMYB19 over-expressing plants plants plants. Collectively, the over-expression of CmMYB19 restricted the multiplication of the aphids on the host, mediated by an enhanced accumulation of lignin.
从菊花中分离出了编码MYB（v-myb禽成髓细胞瘤病毒癌基因同源物）转录因子CmMYB19的基因。它编码一个由200个氨基酸组成的蛋白质，属于R2R3-MYB亚家族。CmMYB19在酵母中未被转录激活，而在洋葱表皮细胞中进行的瞬时表达实验表明，CmMYB19产物定位于细胞核。蚜虫（桑氏毛管蚜）侵染可诱导CmMYB19转录，且叶和茎中的转录本丰度高于根。CmMYB19的过表达限制了蚜虫的繁殖。对参与木质素合成的主要基因转录本丰度的比较表明，CmPAL1（苯丙氨酸解氨酶1）、CmC4H（肉桂酸4-羟化酶）、Cm4CL1（4-羟基肉桂酰辅酶A连接酶1）、CmHCT（羟基肉桂酰辅酶A-莽草酸/奎尼酸羟基肉桂酰转移酶）、CmC3H1（香豆酸3-羟化酶1）、CmCCoAOMT1（咖啡酰辅酶A O-甲基转移酶1）和CmCCR1（肉桂酰辅酶A还原酶1）均上调，这与CmMYB19过表达植株中木质素含量增加一致。总体而言，CmMYB19的过表达通过增强木质素积累介导，限制了宿主上蚜虫的繁殖。
