Bohorquez-Chaux Adriana, Becerra Lopez-Lavalle Luis Augusto, Barrera-Enriquez Vianey, Gómez-Jiménez María Isabel, Sanchez-Sarria Camilo E, Delgado Luis Fernando, Zhang Xiaofei, Gimode Winnie
Alliance of Bioversity International and the International Center for Tropical Agriculture (CIAT), Km 17, Recta Cali-Palmira, A. A. 6713, Cali, Colombia.
International Center for Agricultural Research in the Dry Areas (ICARDA), BP 6202, Rabat, Morocco.
Theor Appl Genet. 2025 Jun 24;138(7):160. doi: 10.1007/s00122-025-04949-1.
QTL associated with whitefly resistance were identified in a cassava F population and KASP markers applicable in selection for the trait were validated. Whitefly species pose a major threat to cassava production in tropical regions causing direct plant damage and transmitting viruses that lead to devastating cassava diseases. Aleurotrachelus socialis whitefly is one of the pests that affect cassava in South America. Developing resistant cassava varieties is the most sustainable control strategy for managing whiteflies. This study aimed to map the quantitative trait loci (QTL) associated with resistance to A. socialis and develop molecular markers to facilitate marker-assisted selection. An F cassava population (N = 183) was generated by selfing a highly resistant F derived from a cross between ECU72 (resistant) and COL2246 (susceptible) landraces. Phenotyping was performed using an efficient glasshouse screening method and high throughput image analysis of infested leaves (Nymphstar). We identified QTL on chromosomes 1, 2, 5, 6, 8, 9, and 14, with a stable and highly significant QTL on chromosome 8 (MeF2WFly8.1), explaining 35.44% of the phenotypic variation. To enable efficient selection, high-throughput KASP markers were developed and validated across diverse genetic backgrounds. Three SNPs displayed the highest association with whitefly resistance, with Chr08_6483145 as the most effective marker for selection in diverse backgrounds. These markers are provided for improving the efficiency of whitefly resistance breeding in the global cassava community.
在一个木薯F群体中鉴定出与粉虱抗性相关的QTL，并验证了适用于该性状选择的KASP标记。粉虱种类对热带地区的木薯生产构成重大威胁，会直接损害植株并传播导致木薯毁灭性病害的病毒。烟粉虱是影响南美洲木薯的害虫之一。培育抗木薯品种是防治粉虱最可持续的策略。本研究旨在定位与抗烟粉虱相关的数量性状位点（QTL），并开发分子标记以促进标记辅助选择。通过自交一个由ECU72（抗性）和COL2246（感病）地方品种杂交产生的高抗性F1植株，获得了一个F木薯群体（N = 183）。使用高效的温室筛选方法和对受侵染叶片（若虫星）的高通量图像分析进行表型分析。我们在第1、2、5、6、8、9和14号染色体上鉴定出QTL，其中第8号染色体上有一个稳定且高度显著的QTL（MeF2WFly8.1），解释了35.44%的表型变异。为了实现高效选择，开发了高通量KASP标记并在不同遗传背景下进行了验证。三个单核苷酸多态性（SNP）与粉虱抗性的关联度最高，其中Chr08_6483145是在不同背景下进行选择的最有效标记。提供这些标记是为了提高全球木薯群体中粉虱抗性育种的效率。
