Hoffmann M K
Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Lymphokine Res. 1987 Fall;6(4):299-308.
The lymphokines Interleukin 1 and 2 play essential roles in the generation of antibody to artificial and natural red cell bound antigens. Critical for the appropriate function of these lymphokines is the concentration of cAMP in responding B lymphocytes. Antigen-reactive B cells mount a strong immune response in the presence of IL-1 and IL-2 when their cAMP is high, but become unresponsive when their cAMP is low. We show here that reagents which raise cAMP concentrations (Prostaglandin E, cholera toxin, forskolin) synergize strongly with IL-1 and IL-2 in the generation of antibody. We searched for physiological signals which synergize with IL-1 and IL-2 in a similar fashion and found that peritoneal macrophages facilitate strong antibody production by B cells. This helper effect is blocked by indomethacin, suggesting that it is mediated by the cyclo-oxygenase pathway and prostaglandin. Helper T cells can also complement IL-1 and IL-2 in stimulating B cells to produce antibody. This effect is not blocked by indomethacin.
淋巴因子白细胞介素1和白细胞介素2在针对人工和天然红细胞结合抗原产生抗体的过程中发挥着重要作用。这些淋巴因子正常发挥功能的关键在于应答性B淋巴细胞中cAMP的浓度。当抗原反应性B细胞内的cAMP水平较高时，在白细胞介素1和白细胞介素2存在的情况下会产生强烈的免疫反应，但当cAMP水平较低时则变得无反应。我们在此表明，提高cAMP浓度的试剂（前列腺素E、霍乱毒素、福斯高林）在抗体产生过程中与白细胞介素1和白细胞介素2具有强烈的协同作用。我们寻找了以类似方式与白细胞介素1和白细胞介素2协同作用的生理信号，发现腹腔巨噬细胞促进B细胞产生大量抗体。这种辅助作用被吲哚美辛阻断，表明它是由环氧化酶途径和前列腺素介导的。辅助性T细胞在刺激B细胞产生抗体方面也能补充白细胞介素1和白细胞介素2的作用。这种作用不会被吲哚美辛阻断。
