Gilbert K M, Hoffmann M K
J Immunol. 1985 Sep;135(3):2084-9.
Dibutyryl cAMP and IL 1 were found to stimulate antigen-specific and polyclonal antibody production when added together to cultures of highly purified B cells. We propose that IL 1 and an elevation in cytoplasmic cAMP represent minimal signal requirements for B cell activation. In contrast to its effect on B cells, dibutyryl cAMP inhibited helper T cell activity. Cyclic AMP suppressed the production of IL 2 and T cell replacing factor (TRF) by T cells and thus abrogated the ability of helper T cells to enhance SRBC-specific antibody production by B cells. Cyclic AMP did not inhibit the generation by T cells of B cell growth factor (BCGF). BCGF, not normally detected in Con A supernatant, was found in the culture supernatant of spleen cells that were stimulated with Con A in the presence of cAMP. Our findings indicate that cAMP blocks the production of an inhibitor of BCGF activity. cAMP had no effect on the production by macrophages of IL 1.
已发现，当将二丁酰环磷腺苷（Dibutyryl cAMP）和白细胞介素1（IL 1）一起添加到高度纯化的B细胞培养物中时，它们会刺激抗原特异性和多克隆抗体的产生。我们提出，白细胞介素1和细胞质中环磷腺苷水平的升高代表了B细胞活化的最低信号要求。与它对B细胞的作用相反，二丁酰环磷腺苷抑制辅助性T细胞活性。环磷腺苷抑制T细胞产生白细胞介素2（IL 2）和T细胞替代因子（TRF），从而消除了辅助性T细胞增强B细胞产生抗绵羊红细胞（SRBC）特异性抗体的能力。环磷腺苷并不抑制T细胞产生B细胞生长因子（BCGF）。在环磷腺苷存在的情况下，用刀豆蛋白A（Con A）刺激的脾细胞培养上清液中发现了通常在Con A上清液中未检测到的BCGF。我们的研究结果表明，环磷腺苷阻断了BCGF活性抑制剂的产生。环磷腺苷对巨噬细胞产生白细胞介素1没有影响。
