Luisetti M, Salmona M, Pozzi E, Genghini M, Spialtini L, Masturzo P
Istituto di Tisiologia e Malattie dell 'Apparato Respiratorio, Università di Pavia, Italy.
Lung. 1987;165(6):333-40. doi: 10.1007/BF02714449.
The influence of pharmacological stimulation of pulmonary surfactant synthesis has been studied in rat alveolar spaces. Animals were treated acutely with Ambroxol at a dose of 4 mg/kg b.w./day p.o. and 5 days later the following biochemical and physico-chemical parameters were determined: BAL fluid lecithin content, BAL fluid microviscosity, alveolar macrophage membrane microviscosity, spontaneous generation of superoxide anion by alveolar macrophages, elastase and antielastase activity of BAL fluid. Treatment with Ambroxol significantly increased the lecithin content of BAL fluid and significantly decreased the macrophage plasma membrane microviscosity. A likely consequence of increased lecithin content in alveolar macrophages (an activation of these cells) was suggested by the increase of the spontaneous production of superoxide. Finally, in the BAL fluid of Ambroxol-treated rats the elastase activity was reduced, whereas the elastase inhibitory activity was almost doubled in respect to control rats.
在大鼠肺泡腔中研究了肺表面活性物质合成的药理刺激作用。动物每天经口给予氨溴索，剂量为4mg/kg体重，连续给药5天，然后测定以下生化和物理化学参数：支气管肺泡灌洗（BAL）液卵磷脂含量、BAL液微粘度、肺泡巨噬细胞膜微粘度、肺泡巨噬细胞超氧阴离子的自发产生、BAL液中的弹性蛋白酶和抗弹性蛋白酶活性。氨溴索治疗显著增加了BAL液中卵磷脂的含量，并显著降低了巨噬细胞质膜的微粘度。超氧自发产生的增加提示肺泡巨噬细胞中卵磷脂含量增加（这些细胞的激活）可能产生的后果。最后，在氨溴索治疗的大鼠的BAL液中，弹性蛋白酶活性降低，而与对照大鼠相比，弹性蛋白酶抑制活性几乎增加了一倍。
