白色念珠菌生物膜检测方法的评估与优化
Assessment and Optimizations of Candida albicans Biofilm Assays.
作者信息
Lohse Matthew B, Gulati Megha, Valle Arevalo Ashley, Fishburn Adam, Johnson Alexander D, Nobile Clarissa J
机构信息
Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA.
Department of Biology, BioSynesis, Inc., San Francisco, California, USA.
出版信息
Antimicrob Agents Chemother. 2017 Apr 24;61(5). doi: 10.1128/AAC.02749-16. Print 2017 May.
Abstract
biofilms have a significant medical impact due to their rapid growth on implanted medical devices, their resistance to antifungal drugs, and their ability to seed disseminated infections. Biofilm assays performed allow for rapid, high-throughput screening of gene deletion libraries or antifungal compounds and typically serve as precursors to studies. Here, we compile and discuss the protocols for several recently published biofilm assays. We also describe improved versions of these protocols as well as novel assays. Finally, we consider some of the advantages and disadvantages of these different types of assays.
摘要
生物膜因其在植入式医疗器械上的快速生长、对抗真菌药物的抗性以及引发播散性感染的能力而具有重大医学影响。所进行的生物膜测定能够对基因缺失文库或抗真菌化合物进行快速、高通量筛选，并且通常作为研究的前奏。在此，我们汇编并讨论了几种最近发表的生物膜测定方法的方案。我们还描述了这些方案的改进版本以及新的测定方法。最后，我们考虑了这些不同类型测定方法的一些优缺点。
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