[采用印迹杂交法检测临床样本中甲型肝炎病毒的基因组RNA]
[Detection of genomic RNA of the hepatitis A virus in clinical samples using the blot hybridization method].
作者信息
Nepliuev I V, Doroshenko N V, Stakhanova V M, Mesianzhinov V V, Zhdanov V M
出版信息
Mol Gen Mikrobiol Virusol. 1987 Sep(9):35-8.
PMID:2828940
Abstract
The synthetic oligonucleotide sequence 3'CTCCTCGTACTTTA-5' complementing hepatitis A virus RNA was compared with cDNA probes in identification of viral genomic RNA. The clinical materials from patients in the 1-2 weeks of jaundice were screened. High specificity of the technique was demonstrated. Possibility of clinical using of the blot hybridization technique is discussed.
摘要
将与甲型肝炎病毒RNA互补的合成寡核苷酸序列3'CTCCTCGTACTTTA-5'与cDNA探针在病毒基因组RNA的鉴定中进行了比较。对黄疸1-2周患者的临床材料进行了筛查。证明了该技术的高特异性。讨论了印迹杂交技术临床应用的可能性。
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