Peretti Leandro E, Gonzalez Verónica D G, Marcipar Iván S, Gugliotta Luis M
INTEC (Universidad Nacional del Litoral and CONICET),Santa Fe (3000),Argentina.
Laboratorio de Tecnología Inmunológica,Facultad de Bioquímica y Ciencias Biológicas,Universidad Nacional del Litoral,Santa Fe (3000),Argentina.
Parasitology. 2017 Jul;144(8):1073-1078. doi: 10.1017/S0031182017000294. Epub 2017 Mar 14.
The aim of this work was to obtain a reagent based on latex particles for ruling out acute toxoplasmosis in pregnant women by immunoagglutination (IA). Latex-protein complexes (LPC) were previously synthesized coupling the recombinant protein of Toxoplasma gondii P22Ag and the homogenate of the parasite to latex particles with different size, chemical functionality and charge density. LPC were tested in IA assays against a panel of 72 pregnant women serum samples. Results were analysed through receiver operating characteristic curves, determining area under the curve (AUC), sensitivity, specificity positive and negative predictive values (PPV and NPV, respectively). It was observed that the antigenicity of proteins was not affected during sensitization by either physical adsorption or covalent coupling. The best results in the sense of maximizing discrimination of low avidity sera from chronic ones were observed for the IA test based on latex particles with carboxyl functionality and the recombinant P22Ag, obtaining an AUC of 0·94, a sensitivity of 100% and a NPV of 100%. In this way, the proposed test could be useful for the toxoplasmosis diagnosis in pregnant women, with the advantages of being cheap, rapid and easy to be implemented.
这项工作的目的是获得一种基于乳胶颗粒的试剂，用于通过免疫凝集（IA）排除孕妇的急性弓形虫病。先前已合成乳胶 - 蛋白质复合物（LPC），将刚地弓形虫P22Ag的重组蛋白和寄生虫匀浆与具有不同大小、化学功能和电荷密度的乳胶颗粒偶联。针对一组72份孕妇血清样本，在IA试验中对LPC进行了测试。通过受试者工作特征曲线分析结果，确定曲线下面积（AUC）、敏感性、特异性、阳性和阴性预测值（分别为PPV和NPV）。观察到，通过物理吸附或共价偶联致敏期间，蛋白质的抗原性不受影响。对于基于具有羧基功能的乳胶颗粒和重组P22Ag的IA试验，在最大程度区分低亲和力血清与慢性血清方面观察到了最佳结果，获得的AUC为0·94，敏感性为100%，NPV为100%。这样，所提出的试验可能对孕妇弓形虫病的诊断有用，具有廉价、快速且易于实施的优点。
