Department of Food Science and Engineering, School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
Department of Food Science and Engineering, School of Marine Sciences, Ningbo University, Ningbo 315211, PR China; Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo University, Ningbo 315211, PR China.
Food Res Int. 2017 Feb;92:9-16. doi: 10.1016/j.foodres.2016.12.008. Epub 2016 Dec 21.
(-)-Epigallocatechin 3-O-(3-O-methyl) gallate (EGCG3″Me) has exhibited multiple beneficial effects on the prevention of obesity in oolong tea. However, its absorption is relatively low, and the potential to be fully utilized is not completely elucidated. Therefore, with human flora-associated (HFA) mice model, the effect of EGCG3″Me on high fat diet-induced obesity was investigated by high-throughput sequencing. The shifts in relative abundance of the dominant taxa at the phylum, family and genus levels showed the dramatically effects of EGCG3″Me. Despite significant inter-individual variation, a large increase in Bacteroidetes with concomitant decrease of Firmicutes was observed after the administration of EGCG3″Me for 8weeks, with a corresponding decrease in the Firmicutes/Bacteroidetes ratio, which reflect the modulatory effect of EGCG3″Me on intestinal microbiota. The results showed that EGCG3″Me may have prebiotic-like activity and can be used as a functional food component with potential therapeutic utility in manipulating intestinal microbiota, contributing to the prevention of gut dysbiosis.
（-）-表没食子儿茶素 3-O-(3-O- 甲基)没食子酸酯（EGCG3″Me）在乌龙茶预防肥胖方面表现出多种有益作用。然而，其吸收相对较低，其被充分利用的潜力尚未完全阐明。因此，本研究利用人源菌群相关（HFA）小鼠模型，通过高通量测序研究了 EGCG3″Me 对高脂肪饮食诱导肥胖的影响。在门、科和属水平上的优势分类群相对丰度的变化显示了 EGCG3″Me 的显著作用。尽管存在显著的个体间变异性，但在给予 EGCG3″Me 治疗 8 周后，观察到拟杆菌门的大量增加，同时厚壁菌门减少，伴随着厚壁菌门/拟杆菌门比值的相应降低，反映了 EGCG3″Me 对肠道微生物群的调节作用。结果表明，EGCG3″Me 可能具有类似益生元的活性，可作为功能性食品成分，具有通过操纵肠道微生物群预防肠道功能紊乱的潜在治疗用途。
