牛白血病病毒前病毒整合到受感染的人骨髓瘤细胞的DNA中。
Provirus integration of bovine leukemia virus into DNA of infected human myeloma cells.
作者信息
Sláviková K, Kettmann R, Reinerová M, Zajac V, Portetelle D, Burny A
机构信息
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
出版信息
Neoplasma. 1987;34(6):653-7.
Sixty clones of bovine leukemia virus-infected B-human myeloma ARH77 cells were isolated. The DNAs of all clones were examined for BLV provirus integration by Southern blotting analysis. Proviral sequences were found in DNAs of two clones. One of them (clone I B3) contained one proviral copy with a deletion of approximately 5.5 kb; the other one (clone I F9) carried three integrated proviruses. Viral proteins of 70,000, 42,000 and 35,000 M. W. were found in extracts of clone I F9. In clone I B3 only the 70,000 M.W. protein was detected.
分离出60个感染牛白血病病毒的B-人骨髓瘤ARH77细胞克隆。通过Southern印迹分析检查所有克隆的DNA中牛白血病病毒前病毒的整合情况。在两个克隆的DNA中发现了前病毒序列。其中一个（克隆I B3）含有一个约5.5 kb缺失的前病毒拷贝；另一个（克隆I F9）携带三个整合的前病毒。在克隆I F9的提取物中发现了分子量为70,000、42,000和35,000的病毒蛋白。在克隆I B3中仅检测到分子量为70,000的蛋白。
Zotero 插件