Luo Hong, Gao Hualin, Liu Fang, Qiu Bing
Department of Orthopaedic Surgery, Guizhou Orthopaedics Hospital, Guiyang, Guizhou 550001, P.R. China.
Department of Anesthesiology, Guizhou Orthopaedics Hospital, Guiyang, Guizhou 550001, P.R. China.
Int J Mol Med. 2017 Apr;39(4):1046-1052. doi: 10.3892/ijmm.2017.2918. Epub 2017 Mar 10.
Currently, the use of stem cell transplantation for the treatment of osteoporosis is a popular research topic. In general, promotion of the osteogenic differentiation of stem cells is the goal of researchers. MicroRNAs (miRNAs of miRs) are critical regulatory factors of osteogenic induction, and miR‑9 and miR‑10 have been substantiated to be involved in the osteogenic process, yet the underlying mechanisms remain unclear. The present study investigated the role of miR‑9 and miR‑10 in the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) and aimed to elucidate the mechanism of osteogenic differentiation. Using MTT assay and RT-PCR, we found that miR‑9 expression was upregulated and miR-10 expression was downregulated during osteogenic differentiation. Moreover, as shown by the results of mineralization analysis and western blot analysis, the overexpression of miR‑9 enhanced the osteogenic abilities and overexpression of miR‑9 inhibited these abilities. Furthermore, miR‑9 and miR‑10 also affected the expression of Runt‑related transcription factor 2 (Runx2) and extracellular signal-regulated kinase (ERK) pathway by western blot analysis. Based on these results, we conclude that miR‑9 and miR‑10 play key roles in regulating the differentiation of BMSCs.
目前，使用干细胞移植治疗骨质疏松症是一个热门的研究课题。一般来说，促进干细胞的成骨分化是研究人员的目标。微小RNA（miRNA或miR）是成骨诱导的关键调节因子，并且已经证实miR-9和miR-10参与成骨过程，但其潜在机制仍不清楚。本研究调查了miR-9和miR-10在骨髓间充质干细胞（BMSC）成骨分化中的作用，并旨在阐明成骨分化的机制。使用MTT法和RT-PCR，我们发现在成骨分化过程中miR-9表达上调而miR-10表达下调。此外，如矿化分析和蛋白质印迹分析结果所示，miR-9的过表达增强了成骨能力，而miR-10的过表达抑制了这些能力。此外，通过蛋白质印迹分析，miR-9和miR-10还影响了Runx相关转录因子2（Runx2）和细胞外信号调节激酶（ERK）途径的表达。基于这些结果，我们得出结论，miR-9和miR-10在调节BMSC的分化中起关键作用。
