Department of Chemistry, Washington State University , P.O. Box 644630, Pullman, Washington 99164, United States.
CSIRO Land and Water, Centre for Environment and Life Sciences , Private Bag No. 5, Wembley, Western Australia 6913, Australia.
Environ Sci Technol. 2017 Apr 18;51(8):4210-4219. doi: 10.1021/acs.est.6b05969. Epub 2017 Mar 29.
Despite comparatively low levels of infection, primary amoebic meningoencephalitis (PAM) induced by Naegleria fowleri is extremely lethal, with mortality rates above 95%. As a thermophile, this organism is often found in moderate-to-warm climates and has the potential to colonize drinking water distribution systems (DWDSs). Current detection approaches require days to obtain results, whereas swift corrective action can maximize the benefit of public health. Presently, there is little information regarding the underlying in situ metabolism for this amoeba but the potential exists to exploit differentially expressed metabolic signatures as a rapid detection technique. This research outlines the biochemical profiles of selected pathogenic and nonpathogenic Naegleria in vitro using an untargeted metabolomics approach to identify a panel of diagnostically meaningful compounds that may enable rapid detection of viable pathogenic N. fowleri and augment results from traditional monitoring approaches.
尽管感染水平相对较低，但福氏耐格里阿米巴原虫引起的原发性阿米巴脑膜脑炎（PAM）的致死率极高，超过 95%。作为一种嗜热生物，该生物体通常存在于中等至温暖的气候中，并有可能在饮用水分配系统（DWDS）中定植。目前的检测方法需要数天才能得出结果，而迅速采取纠正措施可以最大限度地发挥公共卫生的效益。目前，关于这种变形虫的原位代谢的信息很少，但有可能利用差异表达的代谢特征作为一种快速检测技术。本研究使用非靶向代谢组学方法对选定的致病性和非致病性纳格里阿米巴进行了生化特征描述，以鉴定一组具有诊断意义的化合物，这些化合物可能能够快速检测到有活力的致病性福氏耐格里阿米巴，并增强传统监测方法的结果。
