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易位的c-myc基因发生强烈的转录激活,而无需附近有强增强子或启动子。

Strong transcriptional activation of translocated c-myc genes occurs without a strong nearby enhancer or promoter.

作者信息

Kakkis E, Mercola M, Calame K

机构信息

Department of Biological Chemistry, UCLA School of Medicine 90024.

出版信息

Nucleic Acids Res. 1988 Jan 11;16(1):77-96. doi: 10.1093/nar/16.1.77.

Abstract

We have studied the transcriptional activation of translocated c-myc genes in murine plasmacytomas in which the translocation juncture occurs within the first intron of c-myc and juxtaposes c-myc with the immunoglobulin C alpha gene segment. It has been widely suggested that a novel transcriptional enhancer element located near the C alpha gene segment might activate the translocated c-myc gene. We have carried out an extensive search for such an element and find no significant transcriptional enhancer activity in a 22 kb region encompassing the translocation junction, C alpha gene segment and regions 3' of C alpha. We also find that the cryptic promoter region of the translocated c-myc gene is a very weak promoter of transcription. Despite this evidence against the presence of strong transcriptional regulatory elements, the translocated c-myc gene locus is transcribed at high rates that are 25-greater than 100% of that measured for the highly active immunoglobulin genes in murine plasmacytomas. These data suggest the presence of a novel type of strong activator of transcription in the murine heavy chain locus.

摘要

我们研究了小鼠浆细胞瘤中转位c-myc基因的转录激活情况,在这些肿瘤中,转位连接点位于c-myc的第一个内含子内,并使c-myc与免疫球蛋白Cα基因片段并列。广泛认为,位于Cα基因片段附近的一种新型转录增强子元件可能激活转位的c-myc基因。我们对这样一个元件进行了广泛搜索,发现在一个包含转位连接点、Cα基因片段和Cα基因片段3'端区域的22kb区域内没有显著的转录增强子活性。我们还发现,转位的c-myc基因的隐蔽启动子区域是一个非常弱的转录启动子。尽管有这些证据表明不存在强转录调控元件,但转位的c-myc基因位点的转录速率很高,比小鼠浆细胞瘤中高活性免疫球蛋白基因的转录速率高25至100%。这些数据表明在小鼠重链基因座中存在一种新型的强转录激活剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/215d/334614/535e47ab2d0b/nar00143-0097-a.jpg

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