微小RNA-338-5p通过直接靶向表皮生长因子样模块含黏蛋白样激素1抑制胶质母细胞瘤细胞的增殖、迁移、侵袭并促进其凋亡。
MiR-338-5p suppresses proliferation, migration, invasion, and promote apoptosis of glioblastoma cells by directly targeting EFEMP1.
作者信息
Lei Deqiang, Zhang Fangcheng, Yao Dongxiao, Xiong Nanxiang, Jiang Xiaobing, Zhao Hongyang
机构信息
Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China.
Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China.
出版信息
Biomed Pharmacother. 2017 May;89:957-965. doi: 10.1016/j.biopha.2017.01.137. Epub 2017 Mar 9.
OBJECTIVE
We aimed to investigate the effect of miR-338-5p on proliferation, migration and invasion of glioblastoma (GBM) cells by regulating EFEMP1.
METHODS
The expression of miR-338-5p and EFEMP1 was measured by qRT-PCR and western blot. Transfection was conducted to regulate the expression of miR-338-5p and EFEMP1 in U87 cell lines. Cell proliferation, apoptosis, migration and invasion were evaluated using CCK-8 assay, flow cytometry and Transwell assay respectively. Dual luciferase reporter assay was performed to verify whether miR-338-5p directly targeted EFEMP1.
RESULTS
MiR-338-5p was significantly down-regulated in human GBM tumor tissues and cells while EFEMP1 was strongly upregulated (P<0.05). Upregulated miR-338-5p was able to suppress cell proliferation, migration, invasion, and promote cell apoptosis in GBM cells (P<0.05). Dual luciferase reporter gene assay determined that miR-338-5p directly targeted EFEMP1 (P<0.05).
CONCLUSIONS
MiR-338-5p suppressed proliferation, migration and invasion of GBM cells through inhibiting EFEMP1.
目的
我们旨在通过调节EFEMP1来研究miR - 338 - 5p对胶质母细胞瘤（GBM）细胞增殖、迁移和侵袭的影响。
方法
采用qRT - PCR和蛋白质免疫印迹法检测miR - 338 - 5p和EFEMP1的表达。进行转染以调节U87细胞系中miR - 338 - 5p和EFEMP1的表达。分别使用CCK - 8法、流式细胞术和Transwell法评估细胞增殖、凋亡、迁移和侵袭情况。进行双荧光素酶报告基因检测以验证miR - 338 - 5p是否直接靶向EFEMP1。
结果
miR - 338 - 5p在人GBM肿瘤组织和细胞中显著下调，而EFEMP1则强烈上调（P<0.05）。上调miR - 338 - 5p能够抑制GBM细胞的增殖、迁移、侵袭，并促进细胞凋亡（P<0.05）。双荧光素酶报告基因检测确定miR - 338 - 5p直接靶向EFEMP1（P<0.05）。
结论
miR - 338 - 5p通过抑制EFEMP1抑制GBM细胞的增殖、迁移和侵袭。
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