Toniolo D, Persico M, Alcalay M
International Institute of Genetics and Biophysics, Consiglio Nazionale delle Richerche, Naples, Italy.
Proc Natl Acad Sci U S A. 1988 Feb;85(3):851-5. doi: 10.1073/pnas.85.3.851.
An X chromosome gene located 40 kilobases downstream from the G6PD gene, at Xq28, was isolated and sequenced. This gene, which we named GdX, spans about 3.5 kilobases of genomic DNA. GdX is a single-copy gene, is conserved in evolution, and has the features of a "housekeeping" gene. At its 5' end, a cluster of CpG dinucleotides is methylated on the inactive X chromosome and unmethylated on the active X chromosome. The GdX gene can code for a 157 amino acid protein, GdX. Residues 1-74 of GdX show 43% identity to ubiquitin, a highly conserved 76 amino acid protein. The COOH-terminal moiety of GdX is characterized in its central part (residues 110-128) by a sequence homologous to the COOH-terminal hormonogenic site of thyroglobulin. The structural organization of the GdX protein suggests the existence of a family of genes, in addition to the ubiquitin gene, that could play specific roles in key cellular processes, possibly through protein-protein recognition.
一个位于G6PD基因下游40千碱基处、Xq28的X染色体基因被分离并测序。我们将该基因命名为GdX，它跨越约3.5千碱基的基因组DNA。GdX是一个单拷贝基因，在进化过程中保守，具有“管家”基因的特征。在其5'端，CpG二核苷酸簇在失活的X染色体上甲基化，在活性X染色体上未甲基化。GdX基因可编码一种157个氨基酸的蛋白质GdX。GdX的第1 - 74位残基与泛素（一种高度保守的76个氨基酸的蛋白质）有43%的同一性。GdX的COOH末端部分在其中心部分（第110 - 128位残基）具有与甲状腺球蛋白的COOH末端激素生成位点同源的序列。GdX蛋白的结构组织表明，除了泛素基因外，还存在一个基因家族，它们可能通过蛋白质 - 蛋白质识别在关键细胞过程中发挥特定作用。
