一个低拷贝数黏粒。

A low copy number cosmid.

作者信息

Jiang X M, Brahmbhatt H N, Quigley N B, Reeves P R

机构信息

Department of Microbiology, University of Sydney, New South Wales, Australia.

出版信息

Plasmid. 1987 Sep;18(2):170-2. doi: 10.1016/0147-619x(87)90045-x.

DOI:10.1016/0147-619x(87)90045-x

PMID:2829254

Abstract

A low copy number cosmid was constructed by subcloning the pair of cos sites and the kanamycin resistance gene of pcos2EMBL into pGB2. The resulting cosmid, pPR691, has the pSC101 replicon and specifies resistance to kanamycin, spectinomycin, and streptomycin. pPR691 also carries restriction sites suitable for cloning partial Sau3A digests using the strategy of Bates and Swift (P. F. Bates and R. A. Swift, 1983, Gene 26, 137-146). A library of Salmonella typhimurium chromosomal DNA was made using this cosmid and the rfb gene cluster (map position 42) was isolated from this library.

摘要

通过将pcos2EMBL的一对黏粒位点和卡那霉素抗性基因亚克隆到pGB2中构建了一个低拷贝数黏粒。所得的黏粒pPR691具有pSC101复制子，并赋予对卡那霉素、壮观霉素和链霉素的抗性。pPR691还带有适合使用Bates和Swift的策略（P.F. Bates和R.A. Swift，1983，《基因》26卷，第137 - 146页）克隆部分Sau3A消化片段的限制性酶切位点。使用该黏粒构建了鼠伤寒沙门氏菌染色体DNA文库，并从该文库中分离出rfb基因簇（图谱位置42）。