Kirschman J A, Cramer J H
Agrigenetics Advanced Science Company, Madison, WI 53716.
Gene. 1988 Aug 15;68(1):163-5. doi: 10.1016/0378-1119(88)90609-9.
We have constructed two new multi-purpose cloning vectors, pJKKmf(-) and pJKSp/Smf(-), that carry resistance to kanamycin (Km) and spectinomycin/streptomycin (Sp/Sm), respectively. These plasmids are based on pGEM3Zf(-) and therefore contain a pUC-vector-derived multiple cloning site for 13 restriction sites flanked by T7 and SP6 RNA polymerase promoters, the alpha-peptide coding region of beta-galactosidase for blue/white colony screening and the bacteriophage f1 origin of replication for production of single-stranded DNA in the presence of a helper phage. We have used these vectors to reclone sequences from a maize genomic library, to produce radiolabeled RNA probes and to make single-stranded DNA.
我们构建了两种新型多用途克隆载体，即pJKKmf(-)和pJKSp/Smf(-)，它们分别携带对卡那霉素（Km）和壮观霉素/链霉素（Sp/Sm）的抗性。这些质粒基于pGEM3Zf(-)，因此包含一个源自pUC载体的多克隆位点，该位点有13个限制酶切位点，两侧为T7和SP6 RNA聚合酶启动子、用于蓝/白菌落筛选的β-半乳糖苷酶α-肽编码区以及用于在辅助噬菌体存在下生产单链DNA的噬菌体f1复制起点。我们已使用这些载体从玉米基因组文库中重新克隆序列、生产放射性标记的RNA探针并制备单链DNA。
