Department of Chemistry, Faculty of Science, K. N. Toosi University of Technology, Tehran, Iran.
Department of Marine Living Science, Ocean Sciences Research Center, Iranian National Institute for Oceanography and Atmospheric Science, Tehran, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Jun 5;180:204-210. doi: 10.1016/j.saa.2017.03.020. Epub 2017 Mar 7.
A simple and sensitive approach was demonstrated for detection of ascorbic acid (AA) based on seed-mediated growth of silver nanoparticles (Ag NPs). According to the seeding strategy, silver ions existing in the growth solution were reduced to silver atoms on the surface of silver seeds via redox reaction between silver ions and AA. This process -led to appear an absorption band in near 420nm owing to the localized surface plasmon resonance peak of the generated Ag NPs. This change in absorption spectra of Ag NPs caused a change in color of the mixture from colorless to yellow. It was found that the changes in absorption intensity at 420nm have a good relationship with the concentration of AA. Also, detection of AA was achieved through the established colorimetric sensor in the range of 0.25-25μM with detection limit of 0.054μM. Moreover, the selectivity of the method was evaluated with considering potential interferences. The method showed high selectivity toward AA rather than potential interferences and coexisted molecules with AA. It was successfully applied for detection and determination of AA in pharmaceutical tablets and commercial lemonade.
一种简单灵敏的方法被提出，用于基于银纳米粒子（Ag NPs）的种子介导生长来检测抗坏血酸（AA）。根据种子策略，通过银离子和 AA 之间的氧化还原反应，生长溶液中的银离子被还原为银种子表面的银原子。这一过程导致在近 420nm 处出现吸收带，这是由于生成的 Ag NPs 的局域表面等离子体共振峰。Ag NPs 吸收光谱的这种变化导致混合物的颜色从无色变为黄色。结果发现，在 420nm 处的吸收强度变化与 AA 的浓度有很好的关系。此外，通过建立的比色传感器在 0.25-25μM 的范围内实现了对 AA 的检测，检测限为 0.054μM。此外，还通过考虑潜在干扰物来评估该方法的选择性。该方法对 AA 具有很高的选择性，而不是潜在的干扰物和与 AA 共存的分子。该方法成功地用于检测和测定药物片剂和商业柠檬汁中的 AA。
