Borah Nirangkush, Kalita Amlan Jyoti, Guha Ankur Kanti, Das Manash R, Tamuly Chandan
Natural Product Chemistry Section, CSIR-North East Institute of Science and Technology, Branch Itanagar Arunachal Pradesh-791110 India
Academy of Scientific and Innovative Research (AcSIR) Ghaziabad-201002 India.
RSC Adv. 2021 Nov 16;11(58):36698-36706. doi: 10.1039/d1ra04204a. eCollection 2021 Nov 10.
In this work, a colorimetric approach for the detection of ascorbic acid (AA) and thyroxine (TH) was developed by synthesizing cost-effective silver nanoparticles (AgNPs) decorated with epigallocatechin gallate (EGCG) and CTAB. EGCG is the major bioactive chemical constituent that played a significant role in this study. The environment around the nanoparticle (NP) was controlled by adding CTAB surfactants. The synthesized NPs were characterized by different advanced techniques including XRD, XPS, SEM, and TEM. UV-visible spectra were thoroughly analyzed for sensing of AA and TH and the colour change of the solution can be visually monitored. The change in the localized surface plasmon resonance (LSPR) properties was used as an asset for the detection of AA and TH. A good linear relationship was obtained in both the sensing schemes with a limit of detection (LoD) of 0.67 μM and 0.33 μM for AA and TH respectively. Furthermore, the nanoparticles (NP) were implemented for real-sample analysis (pharmaceutical tablets). A cost-effective filter paper strip-based method coupled with smartphone scanning sensing was developed for the detection of AA. The interaction of AA and TH with the probe was depicted by a density functional theory (DFT) analysis. The synthesized NPs show tremendous selectivity towards AA and TH and excellent potential for practical applications.
在本工作中，通过合成用表没食子儿茶素没食子酸酯（EGCG）和十六烷基三甲基溴化铵（CTAB）修饰的具有成本效益的银纳米颗粒（AgNP），开发了一种用于检测抗坏血酸（AA）和甲状腺素（TH）的比色法。EGCG是在本研究中起重要作用的主要生物活性化学成分。通过添加CTAB表面活性剂来控制纳米颗粒（NP）周围的环境。通过包括XRD、XPS、SEM和TEM在内的不同先进技术对合成的NP进行了表征。对紫外可见光谱进行了全面分析以检测AA和TH，并且溶液的颜色变化可以通过肉眼监测。局部表面等离子体共振（LSPR）特性的变化被用作检测AA和TH的依据。在两种传感方案中均获得了良好的线性关系，AA和TH的检测限（LoD）分别为0.67 μM和0.33 μM。此外，将纳米颗粒（NP）用于实际样品分析（药物片剂）。开发了一种基于滤纸条的具有成本效益的方法，并结合智能手机扫描传感用于检测AA。通过密度泛函理论（DFT）分析描述了AA和TH与探针的相互作用。合成的NP对AA和TH表现出极大的选择性以及出色的实际应用潜力。
