Sadir A M, Sardi S, Ruiz M L, Blanco Viera F J, Bolondi A, Parraud J R, Schudel A A
Departamento de Virología, Instituto Nacional de Tecnología Agropecuaria, Castelar, Argentina.
Rev Argent Microbiol. 1985;17(1):15-9.
An inactivated vaccine was prepared with Parainfluenza-3 virus strain LQ-514 and strains of Pasteurella hemolytica and P. multocida, suspended in oil adjuvant. The virus had been isolated from 30-60 day old calves during an epidemic of Pneumonia. The vaccine was tested in guinea pigs aged 1 to 2 months. The antibody response and the virus titres in organs after the challenge were the parameters studied. Hemagglutination inhibition antibodies were first detected 14 days after vaccination and reached maximum titres at day 28. The challenge was done at day 34, and a secondary antibody response was observed 72 hours later, which reached its peak the following day. Virus could be isolated from lung samples of control animals at day 3, 4 and 5 after infection. Moreover, viral antigens and particles were observed in the same samples by immunofluorescence and electron microscopy, respectively. In contrast, all three methods failed to demonstrate the presence of virus in organs of immunized guinea pigs after the challenge.
用副流感 3 型病毒株 LQ - 514 以及溶血巴斯德氏菌和多杀性巴氏杆菌菌株制备了一种灭活疫苗，将其悬浮于油佐剂中。该病毒是在一次肺炎流行期间从 30 - 60 日龄的犊牛中分离出来的。该疫苗在 1 至 2 月龄的豚鼠身上进行了测试。研究的参数是攻毒后抗体反应和器官中的病毒滴度。接种疫苗 14 天后首次检测到血凝抑制抗体，在第 28 天达到最高滴度。在第 34 天进行攻毒，72 小时后观察到二次抗体反应，次日达到峰值。感染后第 3、4 和 5 天可从对照动物的肺样本中分离出病毒。此外，分别通过免疫荧光和电子显微镜在相同样本中观察到病毒抗原和病毒颗粒。相比之下，攻毒后在免疫豚鼠的器官中，所有三种方法均未能证明病毒的存在。
