Pavlov V, Tsvetkov P, Kalcheva M
Vet Med Nauki. 1987;24(9):17-21.
Studied was the immune response of calves, with colostrum antibodies and without colostrum antibodies, immunised with different dosages of live bivalent vaccine against P. parainfluenzae-3 and Adenovirus-1. For the group of calves with colostrum immunity, after single hypodermic immunisation with doses from 10(4) till 10(5) CPE50/cm3 for adenovirus, was not proved immune response, as a result of blocking of the vaccinal antigen of colostrum antibodies. For calves which missed these antibodies, the immune response was determined at the 15th day after the first vaccination with the lesser dose of bivaccine. Regardless of the degree of the antibodies of the calves, after the second vaccination (with the big and the little dose) was proved immune response, which does not differ considerably for the separated groups of calves. It was established, that the optimal dose, necessary for the calves to build an immunity at an early age (from 1 to 3 months), when immunised with bivaccine, was 10(4) CPE50/cm3 for the P. parainfluenzae-3 and 10(3) CPE50/cm3 for the Adenovirus-1. The bigger dose is useless not only from the economic point of view, but from the danger of provoking an unwanted shock of the vaccinated calves.
研究了初乳中有抗体和初乳中无抗体的犊牛，用不同剂量的抗副流感嗜血杆菌3型和腺病毒1型活二价疫苗免疫后的免疫反应。对于有初乳免疫力的犊牛组，用10⁴至10⁵ CPE50/cm³的腺病毒剂量进行单次皮下免疫后，由于初乳抗体对疫苗抗原的阻断作用，未证明有免疫反应。对于缺乏这些抗体的犊牛，在首次用较小剂量二价疫苗接种后的第15天测定免疫反应。无论犊牛抗体程度如何，第二次接种（大剂量和小剂量）后均证明有免疫反应，不同组的犊牛之间差异不大。已确定，犊牛在早期（1至3个月）用二价疫苗免疫时建立免疫力所需的最佳剂量，对于副流感嗜血杆菌3型为10⁴ CPE50/cm³，对于腺病毒1型为10³ CPE50/cm³。大剂量不仅从经济角度来看无用，而且存在引发接种犊牛不必要休克的危险。
