Eichelmann F, Rudovich N, Pfeiffer A F, Schulze M B, Giuseppe R D, Boeing H, Aleksandrova K
Nutrition, Immunity and Metabolism Start-up Lab, Department of Epidemiology, German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), Nuthetal, Germany.
Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, DIfE, Nuthetal, Germany.
Int J Obes (Lond). 2017 Jun;41(6):976-981. doi: 10.1038/ijo.2017.68. Epub 2017 Mar 15.
Adipokines could pose a link between adiposity, systemic inflammation and metabolic disease risk. However, it is unclear whether representative biomarkers are methodologically suitable for use in human obesity research.
We assessed the intra-individual reproducibility of selected adipokines in a sample of 207, apparently healthy, participants with available biosample collections over a 4-month period. Concentrations of the following adipokines were measured at each sampling time point: fatty-acid binding protein-4 (FABP-4), lipocalin-2, monocyte chemoattractant protein 1 (MCP-1), procalcitonin, progranulin, vaspin and visfatin/Nampt. We calculated intraclass correlation coefficients (ICC) and examined Bland-Altman plots.
The analyses suggested an overall good to excellent biomarker reproducibility over 4 months: FABP-4: ICC=0.73 (95% confidence interval: 0.65, 0.78), lipocalin-2: 0.64 (0.55, 0.71), MCP-1: 0.85 (0.81; 0.89), procalcitonin: 0.78 (0.72, 0.83), progranulin: 0.59 (0.50, 0.68) and vaspin: 0.86 (0.82, 0.89). A good agreement of the repeated measurements was further supported by the Bland-Altman plots. No substantial differences in biomarker performance according to adiposity status could be observed. Reliability of visfatin/Nampt could not be assessed due to a high number of measurements below the lower limit of detection.
Results suggest that single measurements of the evaluated adipokines could be used in population-based studies aimed to assess links between obesity, inflammation and metabolic diseases.
脂肪因子可能是肥胖、全身炎症和代谢疾病风险之间的一个联系环节。然而，尚不清楚代表性生物标志物在方法学上是否适用于人类肥胖研究。
我们评估了207名表面健康且有生物样本收集的参与者在4个月期间内所选脂肪因子的个体内重复性。在每个采样时间点测量以下脂肪因子的浓度：脂肪酸结合蛋白-4（FABP-4）、lipocalin-2、单核细胞趋化蛋白1（MCP-1）、降钙素原、前颗粒蛋白、内脏脂肪素和内脂素/烟酰胺磷酸核糖转移酶（Nampt）。我们计算了组内相关系数（ICC）并检查了布兰德-奥特曼图。
分析表明，在4个月期间，生物标志物的重复性总体良好至优秀：FABP-4：ICC = 0.73（95%置信区间：0.65，0.78），lipocalin-2：0.64（0.55，0.71），MCP-1：0.85（0.81；0.89），降钙素原：0.78（0.72，0.83），前颗粒蛋白：0.59（0.50，0.68），内脏脂肪素：0.86（0.82，0.89）。布兰德-奥特曼图进一步支持了重复测量的良好一致性。未观察到生物标志物性能根据肥胖状态有实质性差异。由于大量测量值低于检测下限，无法评估内脂素/Nampt的可靠性。
结果表明，所评估的脂肪因子的单次测量可用于旨在评估肥胖、炎症和代谢疾病之间联系的基于人群的研究。
