Viciani Elisa, Montagnani Francesca, Tordini Giacinta, Romano Antonio, Salerni Lorenzo, De Luca Andrea, Ruggiero Paolo, Manetti Andrea G O
GSK Vaccines S.r.l.Siena, Italy; Host-Microbiota Interaction Team, Wellcome Trust Sanger InstituteCambridge, UK.
Department of Medical Biotechnologies, University of SienaSiena, Italy; Hospital Department of Specialized and Internal Medicine, University Division of Infectious DiseasesSiena, Italy.
Front Microbiol. 2017 Feb 28;8:294. doi: 10.3389/fmicb.2017.00294. eCollection 2017.
Recently we reported an association between pediatric obstructive sleep apnea syndrome (OSAS) and Group A (GAS) sub-acute chronic tonsil colonization. We showed that GAS may contribute to tonsil hyperplasia via a streptolysin O (SLO)-dependent cysteinyl leukotrienes (CysLTs) production, which can trigger T and B cell proliferation. In the present study, we characterized the GAS strains isolated from pediatric OSAS patients in comparison with a panel of age and sex matched GAS strains unrelated to OSAS, but isolated in the same area and during the same period ranging from 2009 to 2013. We found that gene, previously reported to be associated to CysLTs production pathway, was significantly associated to GAS OSAS strains. Moreover, the most numerous group (32%) of the GAS OSAS strains belonged to M75 type, and 6 out of 7 of these strains harbored the gene. Multilocus Sequence Typing (MLST) experiments demonstrated that the clone emm75/ST49/ was associated to OSAS cases. In conclusion, we found an association between gene and the GAS OSAS strains, and we showed that the clone emm75/ST49 harboring genes and was exclusively isolated from patients affected by OSAS, thus suggesting that this genotype might be associated to the pathogenesis of OSAS, although further studies are needed to elucidate the possible role of in tonsil hypertrophy development.
最近我们报道了儿童阻塞性睡眠呼吸暂停综合征（OSAS）与A组（GAS）亚急性慢性扁桃体定植之间的关联。我们发现GAS可能通过依赖链球菌溶血素O（SLO）的半胱氨酰白三烯（CysLTs）产生促进扁桃体增生，这可触发T和B细胞增殖。在本研究中，我们对从儿童OSAS患者中分离出的GAS菌株进行了特征分析，并与一组年龄和性别匹配但与OSAS无关的GAS菌株进行比较，这些无关菌株是在2009年至2013年同一地区、同一时期分离得到的。我们发现，先前报道与CysLTs产生途径相关的基因与GAS OSAS菌株显著相关。此外，GAS OSAS菌株中数量最多的一组（32%）属于M75型，其中7株中有6株携带该基因。多位点序列分型（MLST）实验表明，克隆emm75/ST49/与OSAS病例相关。总之，我们发现基因与GAS OSAS菌株之间存在关联，并且我们表明携带基因和的克隆emm75/ST49仅从OSAS患者中分离得到，因此提示该基因型可能与OSAS的发病机制相关，尽管需要进一步研究来阐明在扁桃体肥大发展中的可能作用。
