通过emm基因分型和多位点序列分型对来自韩国和日本的耐红霉素化脓性链球菌进行的流行病学研究。
Epidemiological study of erythromycin-resistant Streptococcus pyogenes from Korea and Japan by emm genotyping and multilocus sequence typing.
作者信息
Takahashi Takashi, Arai Kazuaki, Lee Dong Hyun, Koh Eun Ha, Yoshida Haruno, Yano Hisakazu, Kaku Mitsuo, Kim Sunjoo
机构信息
Laboratory of Infectious Diseases, Kitasato Institute for Life Sciences & Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.
Department of Laboratory Medicine, Gyeongsang National University School of Medicine, Jinju, Korea.
出版信息
Ann Lab Med. 2016 Jan;36(1):9-14. doi: 10.3343/alm.2016.36.1.9.
BACKGROUND
We determined the epidemiological characteristics of erythromycin (EM)-resistant Streptococcus pyogenes (group A streptococci, GAS) strains isolated from Korea and Japan, using emm genotyping and multilocus sequence typing (MLST).
METHODS
Clinical isolates of GAS had been collected from 1992 to 2012 in Korea and from 2004 to 2009 in Japan. EM resistance was determined by the microdilution method, and resistance genotypes were assessed by PCR. The emm genotyping and MLST were performed by DNA sequencing.
RESULTS
The emm genotypes and sequence types (STs) were concordant in 143 (85.1%) of 168 EM-resistant GAS strains from Korea. ST36/emm12 (35.1%), ST52/emm28 (22.6%), and ST49/emm75 (16.1%) were the most common types. Most of the ST36 (93.9%) and ST52 (95.8%) strains harbored erm(B), whereas strains ST49, ST42, and ST15 contained mef(A). The concordance between emm genotypes and STs was 41 (93.2%) among 44 EM-resistant GAS strains from Japan. ST36/emm12 (34.1%), ST49/emm75 (18.2%), and ST28/emm1 (15.9%) were the major types. ST36 isolates harbored either erm(B) (56.3%) or mef(A) (37.5%), whereas isolates ST28, ST49, and ST38 carried only mef(A). The proportion of erm(B) and mef(A) was 66.1% and 33.3% in Korea and 22.7% and 68.2% in Japan, respectively.
CONCLUSIONS
The common STs in Korea and Japan were ST36 and ST49, whereas ST52 was present only in Korea and ST28 only in Japan. Genotype erm(B) was predominant in Korea, whereas mef(A) was frequent in Japan. There were differences between Korea and Japan regarding the frequencies of emm genotypes, STs, and EM resistance genes among the EM-resistant GAS.
背景
我们运用emm基因分型和多位点序列分型（MLST）方法，确定了从韩国和日本分离出的耐红霉素（EM）的化脓性链球菌（A组链球菌，GAS）菌株的流行病学特征。
方法
1992年至2012年在韩国以及2004年至2009年在日本收集了GAS临床分离株。通过微量稀释法测定EM耐药性，并通过PCR评估耐药基因型。通过DNA测序进行emm基因分型和MLST。
结果
来自韩国的168株耐EM的GAS菌株中，有143株（85.1%）的emm基因型和序列类型（STs）一致。ST36/emm12（35.1%）、ST52/emm28（22.6%）和ST49/emm75（16.1%）是最常见的类型。大多数ST36（93.9%）和ST52（95.8%）菌株携带erm(B)，而ST49、ST42和ST15菌株含有mef(A)。在来自日本的44株耐EM的GAS菌株中，emm基因型和STs之间的一致性为41株（93.2%）。ST36/emm12（34.1%）、ST49/emm75（18.2%）和ST28/emm1（15.9%）是主要类型。ST36分离株携带erm(B)（56.3%）或mef(A)（37.5%），而ST28、ST49和ST38分离株仅携带mef(A)。erm(B)和mef(A)在韩国的比例分别为66.1%和33.3%，在日本分别为22.7%和68.2%。
结论
韩国和日本常见的STs是ST36和ST49，而ST52仅在韩国存在，ST28仅在日本存在。基因型erm(B)在韩国占主导地位，而mef(A)在日本较为常见。在耐EM的GAS中，韩国和日本在emm基因型、STs和EM耐药基因的频率方面存在差异。
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