尼古丁对大鼠睾丸组织的损伤；bcl-2、p53和caspase-3表达的证据
Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression.
作者信息
Mosadegh Maryam, Hasanzadeh Shapour, Razi Mazdak
机构信息
Department of Comparative Histology and Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
出版信息
Iran J Basic Med Sci. 2017 Feb;20(2):199-208. doi: 10.22038/ijbms.2017.8249.
OBJECTIVES
Present study was performed in order to uncover new aspects for nicotine-induced damages on spermatogenesis cell lineage.
MATERIALS AND METHODS
For this purpose, 36 mature male Wistar rats were divided into three groups as; control-sham (0.2 ml, saline normal, IP), low dose (0.2 mg/kg BW, IP) nicotine-received and high dose (0.4 mg/kg BW, IP) nicotine-received groups. Following 7 weeks, the expression of bcl-2, p53 and caspase-3 at mRNA and protein levels were investigated by using reverse-transcriptase PCR (RT-PCR) and immunohistochemical (IHC) analyses, respectively. Moreover, the serum level of FSH, LH and testosterone were evaluated. Finally, the mRNA damage was analyzed by using special fluorescent staining.
RESULTS
Nicotine, at both dose levels, decreased tubular differentiation, spermiogenesis and repopulation indices and enhanced cellular depletion. Animals in nicotine-received groups exhibited a significant (<0.05) reduction at mRNA and protein levels of bcl-2. More analyses revealed a remarkable (<0.05) enhancement in expression of p53 and caspase-3 in comparison to control-sham animals. Finally, nicotine resulted in a significant (<0.05) reduction in serum level of testosterone and elevated mRNA damage.
CONCLUSION
Our data showed that, nicotine by suppressing the testosterone biosynthesis, reducing mRNA and protein levels of bcl-2 and up regulating the p53 and caspase-3 mRNA and protein levels adversely affects the spermatogenesis and results in cellular depletion.
目的
进行本研究以揭示尼古丁对生精细胞谱系损伤的新方面。
材料与方法
为此，将36只成熟雄性Wistar大鼠分为三组：对照 - 假手术组（0.2 ml，生理盐水，腹腔注射）、低剂量（0.2 mg/kg体重，腹腔注射）尼古丁处理组和高剂量（0.4 mg/kg体重，腹腔注射）尼古丁处理组。7周后，分别使用逆转录聚合酶链反应（RT-PCR）和免疫组织化学（IHC）分析研究bcl-2、p53和caspase-3在mRNA和蛋白质水平的表达。此外，评估血清中促卵泡激素（FSH）、促黄体生成素（LH）和睾酮的水平。最后，使用特殊荧光染色分析mRNA损伤。
结果
两个剂量水平的尼古丁均降低了生精小管分化、精子发生和再殖指数，并增加了细胞耗竭。尼古丁处理组的动物在bcl-2的mRNA和蛋白质水平上表现出显著（<0.05）降低。更多分析显示，与对照 - 假手术组动物相比，p53和caspase-3的表达显著（<0.05）增强。最后，尼古丁导致血清睾酮水平显著（<0.05）降低和mRNA损伤增加。
结论
我们的数据表明，尼古丁通过抑制睾酮生物合成、降低bcl-2的mRNA和蛋白质水平以及上调p53和caspase-3的mRNA和蛋白质水平，对精子发生产生不利影响并导致细胞耗竭。
Zotero 插件