Khavarimehr Mehdi, Nejati Vahid, Razi Mazdak, Najafi Gholamreza
Department of Biology, Faculty of Basic Science, Urmia University, Urmia, Iran.
Department of Basic Science, Faculty of Veterinary Medicine, Urmia University, P.O. BOX: 1177, Urmia, Iran.
Int Urol Nephrol. 2017 Sep;49(9):1545-1560. doi: 10.1007/s11255-017-1636-5. Epub 2017 Jun 16.
The present study was done to determine the ameliorative effect of polyunsaturated fatty acid omega-3 against experimental diabetes-induced damages on testicular tissue, sperm parameters and preimplantation embryo development in rat model.
Thirty-two mature male rats were divided into two control and test groups. The experimental diabetes (50 mg kg streptozotocin, ip) was induced in test group and subdivided into non-treated diabetic, 300 and 600 mg kg omega-3-treated (orally by gavage) groups. The rats in control group received 0.5 ml saline using intra-gastric gavage. Following 45 days, general histopathological changes, serum level of testosterone, inhibin B, glucose, and sperm parameters, testicular total antioxidant capacity (TAC) and malondialdehyde (MDA) content were analyzed. The mitochondria-dependent apoptosis was investigated by assessing the Bcl-2 and caspase-3 expression as well as DNA fragmentation. Finally, the in vitro fertilization (IVF) potential was examined by evaluating preimplantation embryo developing.
The omega-3 significantly ameliorated the diabetes-induced histological damages, diminished serum level of glucose, testicular MDA content, and enhanced the serum testosterone, inhibin B and testicular TAC. The animals in omega-3-treated groups exhibited a significant (p < 0.05) up-regulation in Bcl-2, as well as remarkable (p < 0.05) down-regulation in caspase-3 expression compared to non-treated diabetic rats. Moreover, the omega-3 maintained DNA integrity, improved sperm quality as well as preimplantation embryo development.
Our data showed that the omega-3 (especially at 600 mg kg dose level) effectively ameliorates the experimental diabetes-induced infertility in rats by up-regulating the testicular endocrine and antioxidant statuses, preventing mitochondria-dependent apoptosis pathway and potentially improving the sperm quality.
本研究旨在确定多不饱和脂肪酸ω-3对实验性糖尿病诱导的大鼠睾丸组织损伤、精子参数及植入前胚胎发育的改善作用。
将32只成熟雄性大鼠分为两个对照组和试验组。试验组诱导实验性糖尿病（腹腔注射50 mg/kg链脲佐菌素），并再细分为未治疗糖尿病组、300 mg/kg和600 mg/kg ω-3治疗组（经口灌胃）。对照组大鼠经胃内灌胃给予0.5 ml生理盐水。45天后，分析一般组织病理学变化、血清睾酮、抑制素B、葡萄糖水平、精子参数、睾丸总抗氧化能力（TAC）和丙二醛（MDA）含量。通过评估Bcl-2和半胱天冬酶-3表达以及DNA片段化来研究线粒体依赖性凋亡。最后，通过评估植入前胚胎发育来检测体外受精（IVF）潜力。
ω-3显著改善了糖尿病诱导的组织学损伤，降低了血清葡萄糖水平、睾丸MDA含量，并提高了血清睾酮、抑制素B和睾丸TAC。与未治疗糖尿病大鼠相比，ω-3治疗组动物的Bcl-2表达显著上调（p < 0.05），半胱天冬酶-3表达显著下调（p < 0.05）。此外，ω-3维持了DNA完整性，改善了精子质量以及植入前胚胎发育。
我们的数据表明，ω-3（尤其是在600 mg/kg剂量水平）通过上调睾丸内分泌和抗氧化状态、防止线粒体依赖性凋亡途径并潜在改善精子质量，有效改善了实验性糖尿病诱导的大鼠不育。
