Binder Brad M
Department of Biochemistry & Cellular and Molecular Biology, M407 Walters Life Sciences, University of Tennessee, 1414 Cumberland Avenue, Knoxville, TN, 37996, USA.
Methods Mol Biol. 2017;1573:211-222. doi: 10.1007/978-1-4939-6854-1_14.
Ethylene is well known to inhibit the growth of dark-grown eudicot seedlings. Most studies examine this inhibition after several days of exposure to ethylene. However, such end-point analysis misses transient responses and the dynamic nature of growth regulation. Here, high-resolution, time-lapse imaging is described as a method to gather data about ethylene growth kinetics and movement responses of the hypocotyls of dark-grown seedlings of Arabidopsis thaliana. These methods allow for the characterization of short-term kinetic responses and can be modified for the analysis of roots and seedlings from other species.
众所周知，乙烯会抑制暗中生长的双子叶植物幼苗的生长。大多数研究在将幼苗暴露于乙烯中数天后检测这种抑制作用。然而，这种终点分析忽略了短暂的反应以及生长调节的动态性质。在此，高分辨率延时成像被描述为一种收集有关拟南芥暗中生长幼苗下胚轴乙烯生长动力学和运动反应数据的方法。这些方法能够表征短期动力学反应，并且可以进行修改以用于分析其他物种的根和幼苗。
