Department of Chemistry, University at Albany, State University of New York , 1400 Washington Avenue, Albany, New York 12222, United States.
Anal Chem. 2017 Apr 4;89(7):4314-4319. doi: 10.1021/acs.analchem.7b00510. Epub 2017 Mar 24.
The Bradford reagent, comprised of the Coomassie Brilliant Blue G-250 dye, methanol, and phosphoric acid, has been traditionally used for quantifying proteins. Use of this reagent in the Bradford assay relies on the binding of the Coomassie Blue G-250 dye to proteins. However, the ability of the dye to react with a small group of amino acids (arginine, histidine, lysine, phenylalanine, tyrosine, and tryptophan) makes it a viable chemical assay for fingerprint analysis in order to identify the biological sex of the fingerprint originator. It is recognized that the identification of biological sex has been readily accomplished using two other methods; however, both of those systems are reliant upon a large group of amino acids, 23 to be precise. The Bradford assay, described here, was developed specifically to aid in the transition from targeting large groups of amino acids, as demonstrated in the previous studies, to targeting only a single amino acid without compromising the intensity of the response and/or the ability to differentiate between two attributes. In this work, we aim to differentiate between female fingerprints and male fingerprints.
考马斯亮蓝 G-250 染料、甲醇和磷酸组成的 Bradford 试剂传统上用于定量蛋白质。Bradford 测定法中使用这种试剂依赖于考马斯亮蓝 G-250 染料与蛋白质的结合。然而，该染料与一小部分氨基酸（精氨酸、组氨酸、赖氨酸、苯丙氨酸、酪氨酸和色氨酸）反应的能力使其成为指纹分析的可行化学测定方法，以识别指纹来源者的生物性别。人们认识到，使用另外两种方法可以很容易地识别生物性别；然而，这两种系统都依赖于一大组氨基酸，确切地说是 23 种。这里描述的 Bradford 测定法是专门开发的，旨在帮助从针对先前研究中显示的大组氨基酸过渡到仅针对单个氨基酸，而不会影响响应的强度和/或区分两种属性的能力。在这项工作中，我们旨在区分女性指纹和男性指纹。
