Department of Breast Surgery, Key Laboratory of Breast Cancer in Shanghai, Fudan University Shanghai Cancer Center, Shanghai 200032, PR China.
Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, PR China.
Sci Rep. 2017 Mar 15;7:44674. doi: 10.1038/srep44674.
Phosphorylated eukaryotic translation initiation factor 2α (p-eIF2α), which functions as a marker of endoplasmic reticulum stress, has been reported to be associated with patient prognosis in various cancers. However, little is known about the prognostic value of p-eIF2α in breast cancer, particularly in different breast cancer subtypes. An immunohistochemistry screen for p-eIF2α was performed using a tissue microarray containing 233 tumors and paired peritumoral tissues from female patients diagnosed with breast cancer. The staining results were scored semiquantitatively, and the p-eIF2α expression level in breast cancer and its potential prognostic value were investigated. In this retrospective cohort study, we found that p-eIF2α levels were significantly upregulated in breast cancer (P < 0.001). p-eIF2α level was negatively correlated with lymph node status (P = 0.039). Survival analysis by Kaplan-Meier estimation and Cox regression showed that p-eIF2α level was correlated with better disease free survival (P = 0.026) and served as an independent prognostic factor (P = 0.046) in patients with triple-negative breast cancer. Our study revealed that p-eIF2α was upregulated in breast cancer and represented a novel predictor of prognosis in patients with triple-negative subtype.
磷酸化真核翻译起始因子 2α（p-eIF2α）作为内质网应激的标志物，已被报道与各种癌症患者的预后相关。然而，关于 p-eIF2α 在乳腺癌中的预后价值，特别是在不同的乳腺癌亚型中，知之甚少。我们使用包含 233 例女性乳腺癌患者肿瘤组织和配对癌旁组织的组织微阵列进行了 p-eIF2α 的免疫组织化学筛选。对染色结果进行半定量评分，研究了乳腺癌中 p-eIF2α 的表达水平及其潜在的预后价值。在这项回顾性队列研究中，我们发现 p-eIF2α 水平在乳腺癌中显著上调（P<0.001）。p-eIF2α 水平与淋巴结状态呈负相关（P=0.039）。Kaplan-Meier 估计和 Cox 回归的生存分析表明，p-eIF2α 水平与无病生存时间相关（P=0.026），并且在三阴性乳腺癌患者中是独立的预后因素（P=0.046）。我们的研究表明，p-eIF2α 在乳腺癌中上调，代表了三阴性亚型患者预后的新预测因子。
