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腺相关病毒载体介导的基因向造血祖细胞的转移。

Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector.

作者信息

LaFace D, Hermonat P, Wakeland E, Peck A

机构信息

Department of Pathology, University of Florida, Gainesville 32610.

出版信息

Virology. 1988 Feb;162(2):483-6. doi: 10.1016/0042-6822(88)90491-6.

DOI:10.1016/0042-6822(88)90491-6
PMID:2829430
Abstract

We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5% by colony formation in the presence of geneticin sulfate (G-418). The value of AAV as an alternative to the retrovirus vector systems is discussed.

摘要

我们在此描述使用重组腺相关病毒（AAV）载体，用显性选择新霉素耐药（Neo）基因转导小鼠造血祖细胞的过程。通过在硫酸遗传霉素（G-418）存在下的集落形成，确定祖细胞成功转化为耐药细胞的比例约为1.5%。文中还讨论了AAV作为逆转录病毒载体系统替代物的价值。

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