Zhang Jing-Kun, Du Run-Xuan, Zhang Lin, Li Ya-Nan, Zhang Ming-Le, Zhao Shuo, Huang Xiang-Hua, Xu Yan-Fang
Department of Obstetrics and Gynecology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, China.
School of Mechanical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, 050018, China.
J Biomed Mater Res A. 2017 Jul;105(7):1949-1959. doi: 10.1002/jbm.a.36066. Epub 2017 Apr 3.
Acellular matrix materials have been widely used to repair various tissues and organs. According to the plastic principle, when a part of the body is lost, it should be replaced with a similar material. Therefore, the use of a homologous organ-specific acellular vaginal tissue in vagina reconstruction repair surgery may show good results. However, the acellular vagina matrix (AVM) form large vertebrates is difficult to isolate. In this study, we described a multistep method to prepare porcine AVM and evaluated the efficacy of acellularization. We also investigated the biomechanical properties, biological activity elements, and biocompatibility of the porcine AVM. We then used this material to reconstruct a rat vagina and performed further morphologic and functional analyses. Small intestinal submucosa (SIS), which is a commonly used acellular matrix material, was used in a control group. Histological examination, DNA content analysis, and agarose gel electrophoresis revealed that the decellularization procedure was effective. The AVM had acceptable biomechanical properties and sufficient growth factor production (VEGF, FGF, TGF-β1, and PDGF-BB) compared with that of the SIS. Subcutaneous transplantation in rats showed that the AVM had good biocompatibility. The tissue-engineered vagina using the AVM more resembled normal-appearing tissue than did that using SIS following morphologic and functional analyses. The AVM has great potential for application in vaginal reconstructive surgery. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1949-1959, 2017.
脱细胞基质材料已被广泛用于修复各种组织和器官。根据塑形原理，当身体的一部分缺失时，应该用类似的材料进行替代。因此，在阴道重建修复手术中使用同源器官特异性脱细胞阴道组织可能会取得良好效果。然而，从大型脊椎动物分离脱细胞阴道基质（AVM）很困难。在本研究中，我们描述了一种制备猪AVM的多步骤方法，并评估了脱细胞效果。我们还研究了猪AVM的生物力学性能、生物活性成分和生物相容性。然后我们使用这种材料重建大鼠阴道，并进行了进一步的形态学和功能分析。对照组使用常用的脱细胞基质材料小肠黏膜下层（SIS）。组织学检查、DNA含量分析和琼脂糖凝胶电泳显示脱细胞过程有效。与SIS相比，AVM具有可接受的生物力学性能和足够的生长因子产生（VEGF、FGF、TGF-β1和PDGF-BB）。大鼠皮下移植显示AVM具有良好的生物相容性。形态学和功能分析后，使用AVM构建的组织工程阴道比使用SIS构建的更接近正常外观组织。AVM在阴道重建手术中具有巨大的应用潜力。©2016威利期刊公司。《生物医学材料研究杂志》A部分：105A：1949 - 1959，2017年。
