Parmaksiz Mahmut, Elçin Ayşe Eser, Elçin Yaşar Murat
Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, and Ankara University Stem Cell Institute, Ankara, Turkey.
Biovalda Health Technologies, Inc., Ankara, Turkey.
Methods Mol Biol. 2018;1577:129-138. doi: 10.1007/7651_2017_33.
Decellularization technology promises to overcome some of the significant limitations in the regenerative medicine field by providing functional biocompatible grafts. The technique involves removal of the cells from the biological tissues or organs for further use in tissue engineering and clinical interventions. There are significant differences between decellularization protocols due to the intrinsic properties of different tissue types and purpose of use. This multistep, chemical-solution-based protocol is optimized for the preparation of decellularized bovine small intestinal submucosa (SIS).
去细胞技术有望通过提供功能性生物相容性移植物来克服再生医学领域中的一些重大限制。该技术涉及从生物组织或器官中去除细胞，以便在组织工程和临床干预中进一步使用。由于不同组织类型的固有特性和使用目的，去细胞方案存在显著差异。这种基于化学溶液的多步骤方案针对去细胞牛小肠黏膜下层（SIS）的制备进行了优化。
