Robertson B H, Brown V K, Bradley D W
Hepatitis Branch, Centers for Disease Control, Atlanta, Georgia 30333.
Virus Res. 1987 Nov;8(4):309-16. doi: 10.1016/0168-1702(87)90003-7.
The nucleotide sequence of the VP1 region of marmoset-attenuated hepatitis A virus (HAV), MS-1, was determined by incorporative dideoxynucleotide sequencing of the RNA obtained from purified, liver-derived virus. Comparison of this nucleotide sequence to those of four previously published isolates revealed that one of the isolates, HM-175, which was obtained from Australia and passed three times in marmosets, had a 8.5-11% nucleotide variability compared to the remaining four isolates which were isolated from North American sources. This nucleotide variability does not result in amino acid differences with the exception of two of the North American isolates, which were derived from tissue culture passage. These isolates have been shown to contain regions of variability generated by nucleotide insertions and/or deletions, while the remaining three isolates, including the Australian isolate, demonstrate limited amino acid differences within the VP1 molecule.
通过对从纯化的肝源性病毒中获得的RNA进行掺入式双脱氧核苷酸测序，确定了狨猴减毒甲型肝炎病毒（HAV）MS-1的VP1区域的核苷酸序列。将该核苷酸序列与之前发表的四个分离株的序列进行比较，发现其中一个分离株HM-175（从澳大利亚获得并在狨猴中传代三次）与其余四个从北美来源分离的分离株相比，核苷酸变异性为8.5%-11%。除了两个来自组织培养传代的北美分离株外，这种核苷酸变异性不会导致氨基酸差异。这些分离株已被证明含有由核苷酸插入和/或缺失产生的可变区域，而其余三个分离株，包括澳大利亚分离株，在VP1分子内显示出有限的氨基酸差异。
