一种自然感染食蟹猴（猕猴属）的新型甲型肝炎病毒的序列分析。

Sequence analysis of a new hepatitis A virus naturally infecting cynomolgus macaques (Macaca fascicularis).

作者信息

Nainan O V, Margolis H S, Robertson B H, Balayan M, Brinton M A

机构信息

Hepatitis Branch, Centers for Disease Control, Atlanta, Georgia 30333.

出版信息

J Gen Virol. 1991 Jul;72 ( Pt 7):1685-9. doi: 10.1099/0022-1317-72-7-1685.

DOI:10.1099/0022-1317-72-7-1685

PMID:1649902

Abstract

A new isolate of hepatitis A virus (HAV), CY-145, was isolated from stool specimens obtained from cynomolgus macaques naturally infected with this agent. Sequence analysis of the capsid region of the genome indicated that this virus differed from other sequenced HAV strains by about 20% at the nucleotide level and 7% at the amino acid level. Two amino acid residues (residues 70 of VP3 and 102 of VP1), previously identified as constituting an immunodominant site and conserved in all sequenced HAVs, were changed in the CY-145 virus. Sequence analysis of a second cynomolgus HAV isolate (CY-55), which came from a different geographical location, showed the same amino acid replacement at these two sites. In addition both isolates had an amino acid substitution at the VP3-VP1 cleavage site. These data suggest that the cynomolgus HAV differs genetically and antigenically from all other sequenced HAVs.

摘要

从自然感染甲型肝炎病毒（HAV）的食蟹猴粪便标本中分离出一种新的HAV分离株CY-145。基因组衣壳区域的序列分析表明，该病毒与其他已测序的HAV毒株在核苷酸水平上相差约20%，在氨基酸水平上相差约7%。CY-145病毒中两个先前被确定为构成免疫显性位点且在所有已测序的HAV中保守的氨基酸残基（VP3的第70位和VP1的第102位）发生了变化。来自不同地理位置的第二种食蟹猴HAV分离株（CY-55）的序列分析显示，这两个位点存在相同的氨基酸替换。此外，两种分离株在VP3-VP1裂解位点都有一个氨基酸替换。这些数据表明，食蟹猴HAV在基因和抗原方面与所有其他已测序的HAV不同。