Meng Kun, Hou Yali, Han Ye, Ban Qiuyan, He Yiheng, Suo Jiangtao, Rao Jingping
College of Horticulture, Northwest A&F University, Yangling 712100, China.
Int J Mol Sci. 2017 Mar 15;18(3):589. doi: 10.3390/ijms18030589.
Lipoxygenase (LOX) initiates the hydroperoxidation of polyunsaturated fatty acids and is involved in multiple physiological processes. In this study, investigation of various microscopic techniques showed that the fruit peel cellular microstructure of the two persimmon cultivars differed after 12 days of storage, resulting in fruit weight loss and an increased number and depth of microcracks. Analysis of subcellular localization revealed that greater amounts of DkLOX3-immunolabelled gold particles accumulated in "Fupingjianshi" than in "Ganmaokui" during storage. In addition, the expression of was positively up-regulated by abscisic acid (ABA), concomitant with the promotion of ethylene synthesis and loss of firmness, and was suppressed by salicylic acid (SA), concomitant with the maintenance of fruit firmness, inhibition of ethylene production and weight loss. In particular, the expression of differed from the ethylene trajectory after methyl jasmonate (MeJA) treatment. Furthermore, we isolated a 1105 bp 5' flanking region of and the activity of promoter deletion derivatives was induced through various hormonal treatments. Promoter sequence -regulatory elements were analysed, and two conserved hormone-responsive elements were found to be essential for responsiveness to hormonal stress. Overall, these results will provide us with new clues for exploring the functions of in fruit ripening and hormonal stress response.
脂氧合酶（LOX）引发多不饱和脂肪酸的氢过氧化反应，并参与多种生理过程。在本研究中，对各种显微技术的研究表明，两个柿品种的果皮细胞微观结构在储存12天后有所不同，导致果实重量减轻以及微裂纹数量和深度增加。亚细胞定位分析显示，在储存期间，“富平尖柿”中积累的DkLOX3免疫标记金颗粒比“甘冒魁”中的更多。此外，[此处原文缺失具体基因名称]的表达受脱落酸（ABA）正向上调，同时促进乙烯合成并导致硬度丧失，而受水杨酸（SA）抑制，同时维持果实硬度、抑制乙烯产生并减轻重量损失。特别是，茉莉酸甲酯（MeJA）处理后，[此处原文缺失具体基因名称]的表达与乙烯轨迹不同。此外，我们分离出了[此处原文缺失具体基因名称]的1105 bp 5'侧翼区域，并且通过各种激素处理诱导了启动子缺失衍生物的活性。对启动子序列调控元件进行了分析，发现两个保守的激素反应元件对于激素应激反应至关重要。总体而言，这些结果将为我们探索[此处原文缺失具体基因名称]在果实成熟和激素应激反应中的功能提供新线索。
