Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
BMC Plant Biol. 2022 Jan 3;22(1):2. doi: 10.1186/s12870-021-03374-y.
MYB Transcription factors (TFs) are most imperative and largest gene family in plants, which participate in development, metabolism, defense, differentiation and stress response. The MYB TFs has been studied in various plant species. However, comprehensive studies of MYB gene family in the sweet cherry (Prunus avium L.) are still unknown.
In the current study, a total of 69 MYB genes were investigated from sweet cherry genome and classified into 28 subfamilies (C1-C28 based on phylogenetic and structural analysis). Microcollinearity analysis revealed that dispersed duplication (DSD) events might play an important role in the MYB genes family expansion. Chromosomal localization, the synonymous (Ks) and nonsynonymous (Ka) analysis, molecular characteristics (pI, weight and length of amino acids) and subcellular localization were accomplished using several bioinformatics tools. Furthermore, the members of distinct subfamilies have diverse cis-acting regions, conserved motifs, and intron-exon architectures, indicating functional heterogeneity in the MYB family. Moreover, the transcriptomic data exposed that MYB genes might play vital role in bud dormancy. The quantitative real-time qRT-PCR was carried out and the expression pattern indicated that MYB genes significantly expressed in floral bud as compared to flower and fruit.
Our comprehensive findings provide supportive insights into the evolutions, expansion complexity and functionality of PavMYB genes. These PavMYB genes should be further investigated as they seem to be brilliant candidates for dormancy manipulation in sweet cherry.
MYB 转录因子（TFs）是植物中最重要和最大的基因家族，参与发育、代谢、防御、分化和应激反应。MYB TFs 在各种植物物种中都有研究。然而，甜樱桃（Prunus avium L.）MYB 基因家族的综合研究尚不清楚。
在本研究中，从甜樱桃基因组中鉴定了 69 个 MYB 基因，并根据系统发育和结构分析将其分为 28 个亚家族（C1-C28）。微共线性分析表明，分散复制（DSD）事件可能在 MYB 基因家族扩张中发挥重要作用。染色体定位、同义（Ks）和非同义（Ka）分析、分子特征（pI、氨基酸的重量和长度）和亚细胞定位使用了几种生物信息学工具完成。此外，不同亚家族的成员具有不同的顺式作用区域、保守基序和内含子-外显子结构，表明 MYB 家族的功能异质性。此外，转录组数据表明 MYB 基因可能在芽休眠中发挥重要作用。进行了定量实时 qRT-PCR，表达模式表明与花和果实相比，MYB 基因在花芽中显著表达。
我们的综合研究结果为 PavMYB 基因的进化、扩张复杂性和功能提供了支持性的见解。这些 PavMYB 基因似乎是甜樱桃休眠操纵的优秀候选基因，应进一步研究。
