Versura P, Maltarello M C, Cellini M, Marinelli F, Caramazza R, Laschi R
Department of Ophthalmology, University of Bologna, Italy.
Acta Ophthalmol (Copenh). 1987 Dec;65(6):661-7. doi: 10.1111/j.1755-3768.1987.tb07060.x.
We characterized the mucus glycoconjugates produced by goblet and non-goblet epithelial cells in asymptomatic contact lens (CL) wearers. We employed four lectins (proteins of vegetal origin which specifically recognize glycosidic residues: WGA, PNA, SBA and ConA) conjugated with colloidal gold as ultrastructural marker, at Transmission Electron Microscopy. A computerized quantitative analysis was carried out in order to compare the results from the CL wearers to those from the control patients. Goblet cells produce different amount of glycosidic residues, in particular, a significant decrease in the distribution of sialic acid, N-acetylglucosamine, N-acetylgalactosamine, galactose-N-acetylgalactosamine and mannose was observed. The content of glycosidic residues in the mucus vesicles of the non-goblet epithelial cells appeared unchanged as to the normal situation. We speculate that the CL could possibly contribute to the failure of the tear film stability by altering the production of mucus.
我们对无症状隐形眼镜佩戴者杯状和非杯状上皮细胞产生的黏液糖缀合物进行了表征。在透射电子显微镜下，我们使用了四种与胶体金偶联的凝集素（植物来源的蛋白质，可特异性识别糖苷残基：小麦胚芽凝集素、花生凝集素、大豆凝集素和刀豆球蛋白A）作为超微结构标记物。进行了计算机定量分析，以便将隐形眼镜佩戴者的结果与对照患者的结果进行比较。杯状细胞产生不同量的糖苷残基，特别是观察到唾液酸、N-乙酰葡糖胺、N-乙酰半乳糖胺、半乳糖-N-乙酰半乳糖胺和甘露糖的分布显著减少。非杯状上皮细胞黏液小泡中糖苷残基的含量与正常情况相比似乎没有变化。我们推测，隐形眼镜可能通过改变黏液的产生而导致泪膜稳定性下降。
