CA 19-9 酶联免疫吸附测定试验：一种研究泪液中黏液变化的新方法。
CA 19-9 ELISA test: a new method for studying mucus changes in tears.
作者信息
Garcher C, Bron A, Baudouin C, Bildstein L, Bara J
机构信息
Department of Ophthalmology, CHU Dijon, France.
出版信息
Br J Ophthalmol. 1998 Jan;82(1):88-90. doi: 10.1136/bjo.82.1.88.
AIMS
This study investigated mucus changes in the tears in various eye conditions using impression cytology. The quantity of mucins was measured by enzyme linked immunosorbent assay (ELISA) using the tumour marker CA 19-9. This assay quantifies the sialylated Lewis(a) structure mainly associated with ocular mucins.
METHODS
Impression cytology was performed using a cellulose nitrate membrane, on 53 healthy patients, 50 glaucoma patients treated with beta blockers, 24 patients suffering from dry eye syndrome, and 45 contact lens wearers. The tear film glycoproteins were eluted and CA 19-9 was measured.
RESULTS
CA 19-9 content expressed as kilo units (kU) per microgram of tears was significantly decreased in dry eye syndrome (25.8 kU (SD 17.3)/microgram) (p < 0.05), glaucoma patients over 60 years (28.9 (19.5) kU/microgram) (p < 0.05), and contact lens wearers (28.4 kU (18)/microgram) (p < 0.05), when compared with healthy individuals (39.4 kU (22.2)/microgram).
CONCLUSION
Impression cytology can be regarded as a valuable method for obtaining samples of glycoconjugates of mucin. The decrease of sialylated chains observed with this method confirms the hypothesis that some quantitative changes in the tear film may be encountered in ocular surface disorders.
目的
本研究采用印迹细胞学方法，调查了各种眼部疾病患者泪液中的黏液变化情况。通过使用肿瘤标志物CA 19-9的酶联免疫吸附测定法（ELISA）来测量黏蛋白的含量。该测定法可对主要与眼部黏蛋白相关的唾液酸化路易斯（a）结构进行定量。
方法
使用硝酸纤维素膜对53名健康受试者、50名接受β受体阻滞剂治疗的青光眼患者、24名患有干眼症综合征的患者以及45名隐形眼镜佩戴者进行印迹细胞学检查。洗脱泪膜糖蛋白并测量CA 19-9。
结果
与健康个体（39.4千单位（标准差22.2）/微克）相比，干眼症综合征患者（25.8千单位（标准差17.3）/微克）（p<0.05）、60岁以上的青光眼患者（28.9（19.5）千单位/微克）（p<0.05）以及隐形眼镜佩戴者（28.4千单位（18）/微克）（p<0.05）泪液中以每微克泪液的千单位（kU）表示的CA 19-9含量显著降低。
结论
印迹细胞学可被视为获取黏蛋白糖缀合物样本的一种有价值的方法。用该方法观察到的唾液酸化链减少证实了这样一种假设，即在眼表疾病中可能会出现泪膜的一些定量变化。
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