Department of Environmental Sciences, University of California, Riverside, CA 92521, USA.
Department of Biological Sciences, University of North Texas, Denton, TX 76203, USA.
Sci Rep. 2017 Mar 15;7:44546. doi: 10.1038/srep44546.
The impacts of Deepwater Horizon (DWH) oil on morphology and function during embryonic development have been documented for a number of fish species, including the economically and ecologically important pelagic species, mahi-mahi (Coryphaena hippurus). However, further investigations on molecular events and pathways responsible for developmental toxicity have been largely restricted due to the limited molecular data available for this species. We sought to establish the de novo transcriptomic database from the embryos and larvae of mahi-mahi exposed to water accommodated fractions (HEWAFs) of two DWH oil types (weathered and source oil), in an effort to advance our understanding of the molecular aspects involved during specific toxicity responses. By high throughput sequencing (HTS), we obtained the first de novo transcriptome of mahi-mahi, with 60,842 assembled transcripts and 30,518 BLAST hits. Among them, 2,345 genes were significantly regulated in 96hpf larvae after exposure to weathered oil. With comparative analysis to a reference-transcriptome-guided approach on gene ontology and tox-pathways, we confirmed the novel approach effective for exploring tox-pathways in non-model species, and also identified a list of co-expressed genes as potential biomarkers which will provide information for the construction of an Adverse Outcome Pathway which could be useful in Ecological Risk Assessments.
已记录了多种鱼类物种（包括经济和生态上重要的洄游性鱼类，鲯鳅）在胚胎发育过程中受深水地平线（Deepwater Horizon，DWH）石油影响的形态和功能变化。然而，由于该物种的分子数据有限，因此对导致发育毒性的分子事件和途径的进一步研究在很大程度上受到限制。我们试图从暴露于两种 DWH 油类型（风化油和源油）的水可容纳馏分（HEWAFs）的鲯鳅胚胎和幼体中建立从头转录组数据库，以努力深入了解特定毒性反应过程中的分子方面。通过高通量测序（HTS），我们获得了鲯鳅的第一个从头转录组，共组装了 60842 个转录本和 30518 个 BLAST 命中。其中，在暴露于风化油后 96hpf 幼体中有 2345 个基因显著调节。通过与参考转录组指导的基因本体论和毒理学途径比较分析，我们证实了该新方法对于探索非模式物种的毒理学途径是有效的，并且还确定了一组共同表达的基因作为潜在的生物标志物，这将为构建对生态风险评估有用的不良结局途径提供信息。
