Ryu Jaewon, Kim Jong M, Lee Chul W, Kim Si W
Department of Energy Convergence, Chosun University, Gwangju, Republic of Korea.
Department of Chemistry, Chonnam National University, Gwangju, Republic of Korea.
J Basic Microbiol. 2017 Jun;57(6):525-535. doi: 10.1002/jobm.201600692. Epub 2017 Mar 10.
A bacterial strain showing strong antifungal activity was isolated from yellow loess and was identified as Paenibacillus kribbensis CU01. Insoluble mucoidal polymers were separated from M9 culture medium via low-speed centrifugation. Most antifungal activity was associated with substances in the insoluble precipitate, which was purified by reverse phase high performance liquid chromatography. Purified fractions were analyzed using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry. Two major ion peaks with mass-to-charge ratio values (m/z) at 883.6 and 897.6 were revealed. After alkaline hydrolysis and sequence analysis, two cyclic depsipeptides were identified as, fusaricidin A and fusaricidin B. Their production was significantly increased by the addition of glucose, Fe , and Mn to M9 medium. Maximum concentrations of produced fusaricidin A and fusaricidin B at flask-scale comprised 460 mg L and 118 mg L , respectively: the highest production concentrations yet reported in the literature. This demonstrates that P. kribbensis CU01 has enormous commercial potential for the mass production of fusaricidin.
从黄土中分离出一株具有强抗真菌活性的细菌菌株，鉴定为克氏类芽孢杆菌CU01。通过低速离心从M9培养基中分离出不溶性黏液聚合物。大部分抗真菌活性与不溶性沉淀物中的物质有关，该沉淀物通过反相高效液相色谱法进行纯化。使用基质辅助激光解吸/电离飞行时间/飞行时间质谱对纯化的馏分进行分析。揭示了两个质荷比（m/z）值分别为883.6和897.6的主要离子峰。经过碱性水解和序列分析，鉴定出两种环缩肽为镰刀菌素A和镰刀菌素B。向M9培养基中添加葡萄糖、铁和锰后，它们的产量显著增加。摇瓶规模下产生的镰刀菌素A和镰刀菌素B的最大浓度分别为460 mg/L和118 mg/L，这是文献中报道的最高生产浓度。这表明克氏类芽孢杆菌CU01在大规模生产镰刀菌素方面具有巨大的商业潜力。
